{"title":"Spectral Study of the Binding of TMetAlPyP3 and ZnTMetAlPyP3 Porphyrins to DNA","authors":"L. R. Aloyan, A. A. Avetisyan","doi":"10.1134/S106833722301005X","DOIUrl":null,"url":null,"abstract":"<p>In this work, according to the data obtained from the induced circular dichroism spectra, the studied porphyrin binds to DNA by the intercalation method, and its Zn-containing analog by the externally ordered interaction was revealed. The absorption spectra of the studied complexes were obtained at different DNA concentrations, and the binding parameters (<i>K</i><sub>b</sub>, <i>n</i>) of porphyrins with DNA were computed from the data at two values of the ionic strength of the solution. It is shown that the places for binding porphyrins become less accessible at a higher ionic strength of the solution. An increase in the melting temperature of DNA in the presence of a metal-containing porphyrin is also observed, as well as a decrease in the stabilizing effect of porphyrins for an increase in the ionic strength of the solution.</p>","PeriodicalId":623,"journal":{"name":"Journal of Contemporary Physics (Armenian Academy of Sciences)","volume":"58 1","pages":"99 - 103"},"PeriodicalIF":0.5000,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Contemporary Physics (Armenian Academy of Sciences)","FirstCategoryId":"101","ListUrlMain":"https://link.springer.com/article/10.1134/S106833722301005X","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSICS, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
In this work, according to the data obtained from the induced circular dichroism spectra, the studied porphyrin binds to DNA by the intercalation method, and its Zn-containing analog by the externally ordered interaction was revealed. The absorption spectra of the studied complexes were obtained at different DNA concentrations, and the binding parameters (Kb, n) of porphyrins with DNA were computed from the data at two values of the ionic strength of the solution. It is shown that the places for binding porphyrins become less accessible at a higher ionic strength of the solution. An increase in the melting temperature of DNA in the presence of a metal-containing porphyrin is also observed, as well as a decrease in the stabilizing effect of porphyrins for an increase in the ionic strength of the solution.
期刊介绍：
Journal of Contemporary Physics (Armenian Academy of Sciences) is a journal that covers all fields of modern physics. It publishes significant contributions in such areas of theoretical and applied science as interaction of elementary particles at superhigh energies, elementary particle physics, charged particle interactions with matter, physics of semiconductors and semiconductor devices, physics of condensed matter, radiophysics and radioelectronics, optics and quantum electronics, quantum size effects, nanophysics, sensorics, and superconductivity.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
