Application of CYP11B2 immunohistochemistry in the histopathological diagnosis of primary aldosteronism

Q4 Medicine

中华内分泌代谢杂志 Pub Date : 2019-11-25 DOI:10.3760/CMA.J.ISSN.1000-6699.2019.11.004

Y. Yi, Xiao Ming, Song Ying, L. Jiayu, Cheng Qingfeng, Yang Shumin, H. Jinbo, Du Zhipeng, L. Qifu

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引用次数: 0

Abstract

Objective To evaluate the value of CYP11B2 immunohistochemistry in the histopathological diagnosis of primary aldosteronism(PA) among Chinese population. Methods Ninety-six cases of unilateral PA patients were retrospectively enrolled, and with 15 cases of non-hyperfunctioning adenoma, and 15 cases of cortisol producing adenoma as control. CYP11B2 immunohistochemistry was performed in the tumor sections of these patients and was analysed by semi-quantified method. The positive rate of CYP11B2 in tumor specimen of all cases enrolled was calculated and PA patients were subtypely classified based on their histopathological features and CYP11B2 expressions. Results The immune-positive rate of CYP11B2 in the PA group was 91.7% (88/96), with the control group being 6.7% (2/30, P<0.001). Subgroup analysis revealed that the immune-positive rate of CYP11B2 in the aldosterone producing adenoma group and unilateral hyperplasia group were 91.8% (78/85) and 90.9% (10/11), respectively. Based upon histopathological feature and CYP11B2 immunohistochemistry, PA patients were further subtypely classified as aldosterone producing adenoma (85/96), unilateral diffuse adrenocortical hyperplasia (7/96), unilateral mono-adrenocortical micronodules (1/96), unilateral multiple adrenocortical micronodules (1/96), and unilateral multiple aldosterone-producing cell clusters (multiple APCCs, 2/96). Conclusion CYP11B2 immunohistochemistry may serve as an useful tool for the histopathological diagnosis of PA. Key words: Primary aldosteronism; Immunohistochemistry; Pathological diagnosis

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CYP11B2免疫组织化学在原发性醛固酮增多症组织病理学诊断中的应用

目的探讨CYP11B2免疫组织化学在中国人群原发性醛固酮增多症（PA）组织病理学诊断中的价值。方法回顾性分析96例单侧PA患者，其中15例为非功能性腺瘤，15例为皮质醇分泌腺瘤。在这些患者的肿瘤切片中进行CYP11B2免疫组织化学，并通过半定量方法进行分析。计算所有入选病例肿瘤标本中CYP11B2的阳性率，并根据其组织病理学特征和CYP11B2表达对PA患者进行亚型分类。结果PA组CYP11B2的免疫阳性率为91.7%（88/96），对照组为6.7%（2/30，P＜0.001），亚组分析显示，醛固酮分泌腺瘤组和单侧增生组的CYP11B2免疫阳性率分别为91.8%（78/85）和90.9%（10/11）。根据组织病理学特征和CYP11B2免疫组织化学，PA患者进一步分型为醛固酮分泌腺瘤（85/96）、单侧弥漫性肾上腺皮质增生（7/96）、单侧单肾上腺皮质微模块（1/96）、，和单侧多个醛固酮产生细胞簇（多个APC，2/96）。结论CYP11B2免疫组化可作为PA组织病理学诊断的有用工具；免疫组织化学；病理学诊断

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来源期刊

中华内分泌代谢杂志 Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

0.60

自引率

0.00%

发文量

7243

期刊介绍： The Chinese Journal of Endocrinology and Metabolism was founded in July 1985. It is a senior academic journal in the field of endocrinology and metabolism sponsored by the Chinese Medical Association. The journal aims to be the "Chinese broadcaster of new knowledge on endocrinology and metabolism worldwide". It reports leading scientific research results and clinical diagnosis and treatment experience in endocrinology and metabolism and related fields, as well as basic theoretical research that has a guiding role in endocrinology and metabolism clinics and is closely integrated with clinics. The journal is a core journal of Chinese science and technology (a statistical source journal of Chinese science and technology papers), and is included in Chinese and foreign statistical source journal databases such as the Chinese Science and Technology Papers and Citation Database, Chemical Abstracts, and Scopus.