Detection of Aflatoxins in Peanut Oils Marketed in Peshawar, Pakistan Using Thin Layer Chromatography

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2021-06-10 DOI:10.18502/jfqhc.8.2.6473

A. Hussain, Z. Rahman, M. Khan

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引用次数: 2

Abstract

Background: Aflatoxins (AFs) are natural toxins produced by fungus belonging to genus Aspergillus. These toxins are the secondary metabolites, which may cause teratogenic, mutagenic, and carcinogenic effects due to contamination of food. Peanut is an economi- cally important crop, grown in many parts of the world. The main aim of this survey was to detect AFs in peanut oils marketed in Peshawar, Pakistan. Methods: During September 2020 to February 2021, a total of 60 peanut oil samples were obtained from retail stores and markets; 20-each from three different areas of Pesh- awar (University, City, and Cantt), Pakistan. AFB1, AFB2, AFG1, and AFG2 were deter- mined using Thin Layer Chromatography. Data analysis was done using SPSS 21.0. Results: Prevalence rates of AFB1, AFB2, AFG1, and AFG2 in peanut oils were 70, 51.7, 3.3, and 0%, respectively. The mean of total AFs was 8.59 μg/kg ranged from 0.12 to 55 μg/kg. Totally, 5% (3 out of 60) of the samples were found contaminated with AFB1 above the permissible limits (20 μg/kg) according to national regulation. There was significant difference (p<0.05) between AF levels in the samples from different three areas of Peshawar. Conclusion: Although, the majority of samples of peanut oils in Peshawar (Pakistan) were safe for consumption, monitoring of AFs must be carried out on a regular basis in the case of peanut oil consumed in this region. This study suggested that farmers, food processors, and local processors should be aware of acceptable hygiene practices for the cultivation, protection, transportation, processing, and handling of peanut oil.

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薄层色谱法检测巴基斯坦白沙瓦市售花生油中的黄曲霉毒素

背景：黄曲霉毒素是由曲霉属真菌产生的天然毒素。这些毒素是次级代谢产物，由于食物污染，可能会引起致畸、致突变和致癌作用。花生是一种重要的经济作物，在世界许多地方都有种植。这项调查的主要目的是检测巴基斯坦白沙瓦市售花生油中的AF。方法：在2020年9月至2021年2月期间，共从零售店和市场获得60份花生油样本；分别来自巴基斯坦Pesh-awar（大学、城市和坎特）三个不同地区的20人。使用薄层色谱法测定AFB1、AFB2、AFG1和AFG2。数据分析采用SPSS 21.0。结果：花生油中AFB1、AFB2、AFG1和AFG2的患病率分别为70%、51.7%、3.3%和0%。总AF的平均值为8.59μg/kg，范围为0.12至55μg/kg。根据国家规定，总共有5%（60%中的3个）的样本被AFB1污染，超过了允许的限度（20μg/kg）。来自白沙瓦不同三个地区的样本中AF水平之间存在显著差异（p<0.05）。结论：尽管白沙瓦（巴基斯坦）的大多数花生油样本是安全消费的，但必须定期对该地区消费的花生油进行AF监测。这项研究表明，农民、食品加工商和当地加工商应了解花生油的种植、保护、运输、加工和处理的可接受的卫生习惯。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.