Toxicity of iron-based nanoparticles to Nannochloropsis oculata: effects of Fe2O3-NPs on oxidative stress and fatty acid composition

IF 1 4区生物学 Q4 ECOLOGY

Marine Biology Research Pub Date : 2023-03-16 DOI:10.1080/17451000.2023.2214400

N. Fazelian, M. Yousefzadi, A. Movafeghi

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引用次数: 0

Abstract

ABSTRACT The growing application of nanotechnology has led to the release of nanoparticles in aquatic ecosystems. Thus, investigating the effect of nanoparticles on microalgae, as the primary producers of the food chain, is an important issue. The present study was conducted to examine the toxicity mechanisms of α- Fe2O3 nanoparticles (Fe2O3-NPs) dominated by oxidative stress in Nannochloropsis oculata. The accumulation of Fe2O3-NPs on the cell surface of N. oculata and in f2 medium was observed in SEM images and DLS analysis, respectively. EDX graph and TEM images also showed the presence of NPs in N. oculata. There was an increase of H2O2 and MDA content and growth inhibition was also elevated in N. oculata exposed to Fe2O3-NPs. Further, Fe2O3-NPs application caused a noticeable enhancement of non-enzymatic antioxidants (carotenoids, phenolic compounds and PUFAs) and APX activity, while the content of SFAs and MUFAs as well as the activity of CAT and PPO were reduced as a result of Fe2O3-NPs stress. Overall, the activation of defence mechanisms was not effective in N. oculata and treatment with concentrations of more than 50 mg l−1 Fe2O3-NPs could induce oxidative stress in this microalga.

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铁基纳米颗粒对眼绿纳米藻的毒性:Fe2O3-NPs对氧化应激和脂肪酸组成的影响

纳米技术的广泛应用导致纳米颗粒在水生生态系统中的释放。因此，研究纳米颗粒对微藻作为食物链的主要生产者的影响是一个重要的问题。本文研究了氧化应激主导的α- Fe2O3纳米颗粒(Fe2O3- nps)对绿纳米藻(Nannochloropsis oculata)的毒性机制。通过扫描电镜(SEM)和DLS分析分别观察到Fe2O3-NPs在N. oculata细胞表面和f2培养基中的积累。EDX图和TEM图也显示了NPs的存在。结果表明，Fe2O3-NPs处理后，红枣的H2O2和MDA含量增加，生长抑制作用增强。此外，Fe2O3-NPs处理显著提高了非酶促抗氧化剂(类胡萝卜素、酚类化合物和PUFAs)和APX活性，降低了SFAs和MUFAs含量以及CAT和PPO活性。总的来说，激活防御机制在N. oculata中不有效，浓度大于50 mg l−1的Fe2O3-NPs处理可以诱导该微藻的氧化应激。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Marine Biology Research 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Marine Biology Research (MBRJ) provides a worldwide forum for key information, ideas and discussion on all areas of marine biology and biological oceanography. Founded in 2005 as a merger of two Scandinavian journals, Sarsia and Ophelia, MBRJ is based today at the Institute of Marine Research, Bergen, Norway. The Journal’s scope encompasses basic and applied research from all oceans and marine habitats and on all marine organisms, the main criterium for acceptance being quality.