Effects of divided injection on the survival of fat graft in nude mice

中华医学美学美容杂志 Pub Date : 2019-02-15 DOI:10.3760/CMA.J.ISSN.1671-0290.2019.01.005

Che LiFeng, Zhenxing Wang, Yuan Li, M. Shan, Jie Yang, Peng Xiao

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Abstract

Objective To explore the effect of divided injection of fat grafting and to provide a new sight for the strategy of clinical particle fat transplantation. Methods Adipose tissue was aspirated from the healthy female abdomen by liposuction. In the control group, 0.5 ml of adipose tissue was subcutaneously injected into the nude mice. The experimental group was injected with 0.25 ml first, followed by 0.25 ml injection of adipose tissue on the 7th, 14th and 30th days. To assess graft retention rate and effectiveness we measured the wet weight and observed the pathological sections with HE or perilipin immunofluorescence. Results The wet weight of the tissue between the experimental group and the control group had no statistical difference, the experimental group had less necrosis and empty tissue than the control group. The proportion of perilipin positive staining tissue in 7 day group had statistical difference from that of the control group. Conclusions The strategy of pre-injection of part of the adipose tissue and then supplement of the residual tissue after 7 days may increase the proportion of active adipose tissue in the graft. Key words: Adipose tissue; Cell transplantation; Animal experiment; Fat granule; Divided injection

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分次注射对裸鼠脂肪移植存活的影响

目的探讨分次注射脂肪移植的效果，为临床颗粒脂肪移植策略提供新的思路。方法采用抽脂法抽吸健康女性腹部脂肪组织。对照组裸鼠皮下注射脂肪组织0.5 ml。实验组先注射0.25 ml，第7天、第14天、第30天再注射0.25 ml脂肪组织。为了评估移植物的保留率和有效性，我们测量了移植物的湿重，并用HE或perilipin免疫荧光观察病理切片。结果实验组与对照组组织湿重比较差异无统计学意义，实验组坏死和空组织少于对照组。7 d组外周血蛋白阳性染色组织比例与对照组比较，差异有统计学意义。结论预先注射部分脂肪组织，7天后补充剩余脂肪组织，可提高移植物中活性脂肪组织的比例。关键词:脂肪组织;细胞移植;动物实验;脂肪粒;分裂的注入

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来源期刊

中华医学美学美容杂志

自引率

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发文量

4641

期刊介绍： "Chinese Journal of Medical Aesthetics and Cosmetology" is a high-end academic journal focusing on the basic theoretical research and clinical application of medical aesthetics and cosmetology. In March 2002, it was included in the statistical source journals of Chinese scientific and technological papers of the Ministry of Science and Technology, and has been included in the full-text retrieval system of "China Journal Network", "Chinese Academic Journals (CD-ROM Edition)" and "China Academic Journals Comprehensive Evaluation Database". Publishes research and applications in cosmetic surgery, cosmetic dermatology, cosmetic dentistry, cosmetic internal medicine, physical cosmetology, drug cosmetology, traditional Chinese medicine cosmetology and beauty care. Columns include: clinical treatises, experimental research, medical aesthetics, experience summaries, case reports, technological innovations, reviews, lectures, etc.