Formulation and Antifungal Activity of Piper betle L. Leaf Extract in Emulsion Gels Against Candida albicans

Majalah Obat Tradisional Pub Date : 2021-04-07 DOI:10.22146/MOT.53257

Widyasari Putranti, Chairisty Asterina, Hardi Astuti Witasari

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Abstract

Candidiasis is the most common fungal infection in humans and is a form of primary and secondary infections of C. albicans. Betel (Piper betle L.) leaf extract has been reported to exhibit efficacious antifungal effects against C. albicans. Emulsion gels, a type of topical dosage form, can deliver hydrophilic and hydrophobic drugs and perform multiple and controlled releases. This research aimed to determine the antifungal activity and physical properties of emulsion gels formulated from betel leaf extract. The dried betel leaves were extracted by maceration with alcohol 95%. Then, with different concentrations (1, 2, and 4%), the extract was formulated into emulsion gels. These dosage forms were later subjected to antifungal activity testing against C. albicans using the cup plate diffusion method that involved Mycoral Cream® for comparison. In this test, the intensity of the activity was determined by measuring the diameter of the formed inhibition zone. The second test evaluated the physical characteristics of the dosage forms, including organoleptic properties, pH, adhesion, dispersion, and viscosity. These data were analyzed using the Mann-Whitney method, and the conclusion was withdrawn from describing the results quantitatively. The reaction yield of the extraction was 9.702%. The analysis results showed that emulsion gels containing 1, 2, and 4% of betel leaf extract created zones of inhibition with diameters of 5.3 ± 0.29, 6.2 ± 0.29, and 10.2 ± 0.41 mm, respectively. As for the physical properties, they differed in pH (6.39 ± 0.120, 6.17 ± 0.132, 5.66 ± 0.123), spreadability (1.849 ± 0.45, 1.816 ± 0.051, 1.771 ± 0.092 g.cm.s-1), adhesion (110 ± 10.8, 126.3 ± 8.5, 142.7 ± 13.50 seconds), and viscosity (2640.35, 1992.95, 2162.12 cps), respectively. The betel leaf emulsion gels exhibited antifungal activity against C. albicans (p <0.05) and met the physical requirements of semi-solid dosage forms.

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花椒叶提取物乳凝胶的配方及对白色念珠菌的抑菌活性研究

念珠菌病是人类最常见的真菌感染，是白色念珠菌的一种原发性和继发性感染。据报道，槟榔叶提取物对白色念珠菌具有有效的抗真菌作用。乳化液凝胶是一种外用剂型，可以给药亲水和疏水药物，并进行多次控释。本研究旨在测定槟榔叶提取物乳液凝胶的抗真菌活性和物理性质。用95%乙醇浸渍法提取槟榔叶。然后，以不同的浓度(1、2和4%)，将提取液配制成乳液凝胶。这些剂型随后使用杯盘扩散法对白色念珠菌进行抗真菌活性测试，并使用Mycoral Cream®进行比较。在本试验中，通过测量形成的抑制带的直径来确定活性的强度。第二个测试评估了剂型的物理特性，包括感官特性、pH值、粘附性、分散性和粘度。使用Mann-Whitney方法对这些数据进行分析，并从定量描述结果中撤回结论。提取物的反应收率为9.702%。结果表明，含1、2、4%槟榔叶提取物的乳液凝胶形成的抑制带直径分别为5.3±0.29、6.2±0.29和10.2±0.41 mm。物理性能方面，pH值(6.39±0.120、6.17±0.132、5.66±0.123)、涂覆性(1.849±0.45、1.816±0.051、1.771±0.092 g.cm.s-1)、附着力(110±10.8、126.3±8.5、142.7±13.50秒)、黏度(2640.35、1992.95、2162.12 cps)差异较大。槟榔叶乳剂凝胶对白色念珠菌具有抗真菌活性(p <0.05)，满足半固体剂型的物理要求。

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来源期刊

Majalah Obat Tradisional

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

15 weeks