Enzymatic disturbances of the Egyptian beetle, Blaps polychresta (Forskål, 1775), as environmental indicators of heavy metal pollution of agricultural soils
Enas E. Nasr , Abdulmohsen I. Algefare , Naif A. Al-Gabri , Zeinab Z. Khater
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引用次数: 3
Abstract
Heavy metals (HMs) pollution is becoming influential severe trouble in the farming ecosystem, where it causes serious health problems for many living organisms. This study was conducted to estimate the HMs accumulation under different sites from agricultural soil and its effects on enzymatic activities in tissues of the beetle, Blaps polychresta, as an indicator of soil contamination. Beetle and soil samples were collected from three agricultural sites; (A) a reference site, (B) fields located near the River Nile, and (C) fields closed to drainage. The atomic absorption results showed a significant increase in iron (Fe), manganese (Mn), arsenic (As), lead (Pb), chromium (Cr), aluminum (Al), and zinc (Zn) for both soil and beetles from site B & C compared to site A. The highest bioaccumulation (BAF) ratio was observed for nickel (Ni), As, and cadmium (Cd) in site C, while Zn, copper (Cu), and Pb in site B. HMs accumulation in beetle's tissues (gut, fat body, and testis) from site B & C induced the malondialdehyde (MDA) activity. Thus, a significant reduction of the antioxidant enzymes and total protein contents (TPc) were observed in beetle tissues from site C. However, no significant differences in some tissues of site B compared to other sites. A principal component analysis (PCA) and correlation were conducted between HMs concentrations in beetle tissues and antioxidant enzymes, TPc, and MDA. The current results of a principal component analysis (PCA) and the correlation test were used to assist the differences in the decline of the antioxidant enzymes: glutathione (GSH), Superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR) and TPc, while; the elevation in MDA responses in beetle's tissues due to HMs accumulations in response to protect themselves from metals toxicity. The antioxidant defense and oxidative stress were more prominent in the tissues of the beetle's gut and fat bodies comparing to the testis. Accordingly, the HMs accumulation in agricultural soil can be predicted by the enzymatic activities in beetle tissues.
重金属污染正在成为农业生态系统的严重问题,对许多生物造成严重的健康问题。本研究旨在研究农用土壤中不同部位的HMs积累量及其对作为土壤污染指标的斑蝽(Blaps polychresta)组织酶活性的影响。从3个农业站点采集甲虫和土壤样本;(A)参考地点,(B)靠近尼罗河的田地,(C)靠近排水的田地。原子吸收结果表明,B点土壤和甲虫中铁(Fe)、锰(Mn)、砷(As)、铅(Pb)、铬(Cr)、铝(Al)和锌(Zn)的含量显著增加;在C点,镍(Ni)、砷(As)和镉(Cd)的生物积累比最高;在B点,锌(Cu)、铜(Pb)的生物积累比最高。C诱导丙二醛(MDA)活性。因此,在c位点的甲虫组织中观察到抗氧化酶和总蛋白含量(TPc)的显著降低,而在B位点的一些组织中与其他位点相比没有显著差异。对甲虫组织中HMs浓度与抗氧化酶、TPc和MDA进行了主成分分析和相关性分析。采用主成分分析(PCA)和相关检验分析了抗氧化酶(谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽- s -转移酶(GST)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)和TPc)下降的差异;甲虫组织中MDA反应的升高是由于HMs的积累,以保护自己免受金属毒性的影响。与睾丸组织相比,甲虫的肠道和脂肪体组织的抗氧化防御和氧化应激更为突出。因此,利用甲虫组织中酶的活性可以预测农业土壤中HMs的积累。
期刊介绍:
Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.