Leaf Nutrient Relations of Cycads in a Common Garden

Abstract

Background and Aims Research required to clarify leaf nutrient relations of cycad species has been inadequate. Common garden studies are useful for determining the influence of genetics on leaf traits because of the homogeneous environment among experimental units. To date, there have been no common garden studies which included all ten genera of cycads. The full phylogenetic breadth has, therefore, not been included in this important area of study. Methods We examined macronutrient and micronutrient content of leaves from one representative species from each of the ten cycad genera at Nong Nooch Tropical Botanical Garden in Thailand. Nitrogen content was determined by dry combustion, and the remaining nutrients were quantified by spectrometry. Results The least variable elements were nitrogen and phosphorus, and the most variable elements were boron and sodium. Nutrient content based on leaflet area was more variable than based on leaflet mass, reflecting species differences in specific leaf area. There were no universal macronutrient or micronutrient signals indicating clear phylogenetic distinctions. Implications for Conservation: Active management of threatened cycad taxa requires research to develop the knowledge to enable evidence-based decisions. This common garden study inclusive of all 10 cycad genera creates a foundation to determine leaf nutrient sufficiency ranges to inform management decisions.