The complete chloroplast genome of Persicaria perfoliata and comparative analysis with Four Medicinal Plants of Polygonaceae.

Abstract

Polygonaceae is a large family of medicinal herbs that includes many species used as traditional Chinese medicine, such as Persicaria perfoliate. Here, we sequenced the complete cp genome of P. perfoliata using Illumina sequencing technology with the purpose to provide a method to facilitate accurate identification. After being annotated, the cp genome of P. perfoliata was compared with Fagopyrum tataricum, Persicaria chinensis, Fagopyrum dibotrys and Fallopia multiflora. The complete cp genome of P. perfoliata is 160,730 bp in length, containing a small single copy (SSC) region of 12,927 bp, a large single copy (LSC) region of 85,433 bp and a pair of inverted repeats (IR) regions of 62,370 bp. A total of 131 genes were annotated, including eight rRNA genes, 34 tRNA genes and 84 protein-coding genes. Forty-two simple sequence repeats and fifty-five repeat sequences were identified. Mutational hot spots analyses indicated that five genes (matK, ndhF, ccsA, cemA, rpl20) could be selected as candidates for molecular markers. Moreover, phylogenetic analysis showed that all the Polygonaceae species formed a monophyletic clade, and P. perfoliata showed the closest relationship with P. chinense. The study provides valuable molecular information to accurately identify P. perfoliata and assist in its development and application.