Extraction of lutein/zeaxanthin from Arthrospira Platensis and optimisation of the saponification process using the response surface methodology

IF 0.9 Q4 ENGINEERING, CHEMICAL
Kevin Joseph Sam, Maya Suresh Nair, S. Velmurugan, R. Rajarathinam, L. Arumugam
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引用次数: 0

Abstract

ABSTRACT This study explores the extraction of lutein and zeaxanthin from Arthrospira Platensis (spirulina), with traditional protocols as a reference and response surface methodology as a tool for optimisation. An enhanced extraction procedure was developed for obtaining higher lutein concentration. The extraction process is further optimised using the central composite method, with the aid of design software tools. Methanol was used as the extracting solvent in a fixed ratio with dry spirulina powder. Saponification and hydrolysis using KOH, water and ethyl acetate/dichloromethane were done for isolating lutein and zeaxanthin from its ester. Ultraviolet–visible spectrophotometry was used for analysing the absorbance of the lutein sample at a wavelength of 445 nm. Using the Response Surface Methodology, trials of various combinations are run for finding optimum conditions of saponification. The major variables were reaction time, reaction temperature and concentration of KOH. Temperature range of 45–75°C, the reaction time of 45–70 min and KOH concentrations ranging from 2% to 10% are observed as the optimum range. The sample is filtered using a PVDF membrane and stored in acetone or isopropyl alcohol. GRAPHICAL ABSTRACT
利用响应面法提取平节螺旋藻叶黄素/玉米黄质并优化皂化工艺
摘要:本研究以传统工艺为参照,以响应面法为优化工具,对平螺旋藻中叶黄素和玉米黄质的提取工艺进行了研究。开发了一种提高叶黄素浓度的提取工艺。在设计软件工具的帮助下,使用中心复合方法进一步优化提取过程。以甲醇为萃取溶剂,与干螺旋藻粉按一定比例萃取。用KOH、水和乙酸乙酯/二氯甲烷进行皂化和水解,从叶黄素酯中分离出叶黄素和玉米黄质。紫外可见分光光度法测定叶黄素样品在445 nm波长处的吸光度。采用响应面法,对不同组合进行了试验,以寻找皂化的最佳条件。主要影响因素有反应时间、反应温度和KOH浓度。最佳反应温度为45 ~ 75℃,反应时间为45 ~ 70 min, KOH浓度为2% ~ 10%。样品用PVDF膜过滤,存放在丙酮或异丙醇中。图形抽象
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来源期刊
Indian Chemical Engineer
Indian Chemical Engineer ENGINEERING, CHEMICAL-
CiteScore
3.00
自引率
6.70%
发文量
33
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