Corrigendum to: Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation

Abstract

Total RNAs of breast cancer cells and mouse embryonic fibroblasts MEF BRCA1 and MEF BRCA1 were extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The cDNA was synthesized with the PrimeScript RT reagent Kit (Promega, Madison, WI, USA). Real-time PCR was carried out using an ABI 7500 real-time PCR system (Applied Biosystems, Foster City, CA, USA). The mRNA expression level of GOT2 and ASS1 was normalized to the endogenous expression of GAPDH. Primers were provided by Invitrogen as described below: