Application of tunable quantum cascade lasers to monitor dynamics of bacteriorhodopsin in the mid-IR spectral range

IF 0.3 Q4 SPECTROSCOPY

Biomedical Spectroscopy and Imaging Pub Date : 2020-05-15 DOI:10.3233/bsi-200195

P. Stritt, Michael Jawurek, K. Hauser

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引用次数: 6

Abstract

The function of membrane proteins is highly impacted by their membrane environment. One suitable approach to get insights into the interaction-induced dynamics of membrane proteins and lipid membranes is time-resolved infrared (IR) spectroscopy. Conclusions about environmental influences to the system can be drawn by correlating the observed kinetics to the well-characterized photocycles of light-driven transmembrane proton pumps like bacteriorhodopsin (BR). For the investigation of photoreceptor-membrane interactions, also minor changes in the absorption spectra must be resolved. Therefore, we applied IR laser spectroscopy using tunable quantum cascade lasers (QCLs) as IR light source. Several QCLs were implemented in a home-built spectrometer and provide a tunability in a broad spectral region covering protein, chromophore and lipid vibrational modes. Kinetics of the BR photocycle were monitored at single wavenumbers. This study demonstrates the high potential of QCL-based spectroscopy for the application to membrane protein studies.

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可调谐量子级联激光器在中红外光谱范围内监测细菌视紫红质动力学中的应用

膜蛋白的功能受到其膜环境的高度影响。深入了解膜蛋白和脂质膜相互作用诱导动力学的一种合适方法是时间分辨红外（IR）光谱。通过将观察到的动力学与光驱动跨膜质子泵（如细菌视紫红质（BR））的良好表征的光循环相关联，可以得出关于环境对系统影响的结论。为了研究光感受器-膜的相互作用，还必须解决吸收光谱中的微小变化。因此，我们使用可调谐量子级联激光器（QCL）作为红外光源来应用红外激光光谱。在自制的光谱仪中实现了几个QCL，并在覆盖蛋白质、发色团和脂质振动模式的宽光谱区域中提供了可调谐性。在单波数下监测BR光循环的动力学。这项研究证明了基于QCL的光谱学在膜蛋白研究中的高潜力。

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Biomedical Spectroscopy and Imaging SPECTROSCOPY-

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期刊介绍： Biomedical Spectroscopy and Imaging (BSI) is a multidisciplinary journal devoted to the timely publication of basic and applied research that uses spectroscopic and imaging techniques in different areas of life science including biology, biochemistry, biotechnology, bionanotechnology, environmental science, food science, pharmaceutical science, physiology and medicine. Scientists are encouraged to submit their work for publication in the form of original articles, brief communications, rapid communications, reviews and mini-reviews. Techniques covered include, but are not limited, to the following: • Vibrational Spectroscopy (Infrared, Raman, Teraherz) • Circular Dichroism Spectroscopy • Magnetic Resonance Spectroscopy (NMR, ESR) • UV-vis Spectroscopy • Mössbauer Spectroscopy • X-ray Spectroscopy (Absorption, Emission, Photoelectron, Fluorescence) • Neutron Spectroscopy • Mass Spectroscopy • Fluorescence Spectroscopy • X-ray and Neutron Scattering • Differential Scanning Calorimetry • Atomic Force Microscopy • Surface Plasmon Resonance • Magnetic Resonance Imaging • X-ray Imaging • Electron Imaging • Neutron Imaging • Raman Imaging • Infrared Imaging • Terahertz Imaging • Fluorescence Imaging • Near-infrared spectroscopy.