Novel Insights Into the Recovery and Stabilization of Rosmarinus officinalis Volatile Aroma Compounds Using Green Solvents

IF 5.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Jelena Vladić, Strahinja Kovačević, Krunoslav Aladić, Silvia Rebocho, Stela Jokić, Sanja Podunavac-Kuzmanović, Ana Rita C. Duarte, Igor Jerković
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Abstract

In this study, the integrated application of supercritical CO2 and natural deep eutectic solvents (NADES) was investigated in order to establish a green procedure that enables obtaining and stabilizing the aroma volatile constituents of Rosmarinus officinalis L. Supercritical CO2 was used to obtain rosemary extracts that possessed an abundance of terpenes, particularly monoterpenes 68.97–88.08% and sesquiterpenes 5.38–21.22%. The obtained extracts were further dispersed in different NADES (betaine/glycerol (Bet/Gly), betaine/ethylene glycol (Bet/EG), and betaine/glycerol/sucrose/water (Bet/Gly/Suc/W) and their stability was assessed at room temperature. The headspace profile of the samples and their antioxidant activity were monitored for 6 months. Changes in the chemical profile of the extract were detected, which corresponded to terpene transformation reactions. In the control (CO2 extract), the development of non-terpene components such as acetic acid was detected, which make the product unsuitable for use. Conversely, the accumulation of acetic acid was not observed in the NADES samples. The antioxidant activity of the control was the most significantly decreased during 6 months, while among the NADES samples, the reduction of activity occurred only in Bet/Gly sample. In Bet/EG and Bet/Gly/Suc/W samples, activity remained the same during the same period. The results suggest that the NADES could serve as stabilization media for CO2-extracted rosemary volatile components. Furthermore, this represents a simple, green process of obtaining readily applicable products with extended stability at room temperature.

用绿色溶剂回收和稳定迷迭香挥发性香气化合物的新见解
本研究调查了超临界二氧化碳和天然深共晶溶剂(NADES)的综合应用,以建立一种绿色程序,从而获得并稳定迷迭香的芳香挥发性成分。超临界二氧化碳用于获得迷迭香提取物,其中含有大量萜烯,尤其是单萜 68.97-88.08% 和倍半萜 5.38-21.22%。得到的提取物进一步分散在不同的 NADES(甜菜碱/甘油(Bet/Gly)、甜菜碱/乙二醇(Bet/EG)和甜菜碱/甘油/蔗糖/水(Bet/Gly/Suc/W))中,并在室温下评估其稳定性。对样品的顶空分布及其抗氧化活性进行了为期 6 个月的监测。检测到萃取物的化学成分发生了变化,这与萜烯转化反应有关。在对照组(二氧化碳提取物)中,检测到乙酸等非萜烯成分的发展,这使得产品不适合使用。相反,在 NADES 样品中没有发现乙酸的积累。在 6 个月期间,对照组的抗氧化活性下降最为明显,而在 NADES 样品中,只有 Bet/Gly 样品的抗氧化活性有所下降。在 Bet/EG 和 Bet/Gly/Suc/W 样品中,活性在同一时期保持不变。结果表明,NADES 可以作为二氧化碳提取的迷迭香挥发性成分的稳定介质。此外,这是一种简单、绿色的工艺,可获得在室温下具有长期稳定性的适用产品。
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来源期刊
Food and Bioprocess Technology
Food and Bioprocess Technology 农林科学-食品科技
CiteScore
9.50
自引率
19.60%
发文量
200
审稿时长
2.8 months
期刊介绍: Food and Bioprocess Technology provides an effective and timely platform for cutting-edge high quality original papers in the engineering and science of all types of food processing technologies, from the original food supply source to the consumer’s dinner table. It aims to be a leading international journal for the multidisciplinary agri-food research community. The journal focuses especially on experimental or theoretical research findings that have the potential for helping the agri-food industry to improve process efficiency, enhance product quality and, extend shelf-life of fresh and processed agri-food products. The editors present critical reviews on new perspectives to established processes, innovative and emerging technologies, and trends and future research in food and bioproducts processing. The journal also publishes short communications for rapidly disseminating preliminary results, letters to the Editor on recent developments and controversy, and book reviews.
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