DAF-16 and SKN-1 mediate Anti-aging and Neuroprotective efficacies of “thai ginseng” Kaempferia parviflora Rhizome extract in Caenorhabditis elegans

Q3 Medicine

Nutrition and Healthy Aging Pub Date : 2022-04-25 DOI:10.3233/nha-210148

M. Prasanth, D. Malar, J. Brimson, Kanika Verma, Aunchalee Tonsomboon, Waluga Plaingam, T. Tencomnao

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引用次数: 1

Abstract

BACKGROUND: The rhizomes of Kaempferia parviflora (KP), have been traditionally used for treating various ailments with 5,7-dimethoxyflavone (DMF) as a prominent compound. OBJECTIVE: To investigate the anti-aging and neuroprotective properties of KP and DMF in Caenorhabditis elegans. METHODS: C. elegans (wild-type (N2), transgenic and mutant strains) were treated with KP and DMF and were monitored for lifespan and neuroprotection through physiological assays, fluorescence microscopy and qPCR analysis. Molecular docking studies were employed to identify the interaction mode of DMF with DAF-16 and SKN-1. RESULTS: KP and DMF significantly increased the lifespan of N2 along with modulating pharyngeal pumping and lipofuscin accumulation. They also exhibited neuroprotection in Aβ transgenic strains by improving lifespan and delaying paralysis. Further, they reduced ROS accumulation significantly in worms exposed to UV-A, thereby exhibiting anti-photoaging potential. KP and DMF could activate SKN-1, DAF-16 which was evident from molecular docking and qPCR analysis. The DAF-2 and DAF-16 mutants did not exhibit any variations in lifespan upon treatment with KP and DMF suggesting the involvement of the DAF-16 mediated pathway in regulating the anti-aging and neuroprotective effects. CONCLUSION: Our findings suggest that KP with DMF as an active ingredient is a potential nutraceutical for aging and associated disorders.

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DAF-16和SKN-1介导“泰参”山奈根提取物对秀丽隐杆线虫的抗衰老和神经保护作用

背景：山奈（KP）的根茎传统上用于治疗各种疾病，5，7-二甲氧基黄酮（DMF）是一种重要的化合物。目的：研究KP和DMF对秀丽隐杆线虫的抗衰老和神经保护作用。方法：用KP和DMF处理秀丽隐杆线虫（野生型（N2）、转基因和突变株），并通过生理测定、荧光显微镜和qPCR分析监测其寿命和神经保护作用。采用分子对接研究来确定DMF与DAF-16和SKN-1的相互作用模式。结果：KP和DMF显著延长了N2的寿命，同时调节了咽泵和脂褐素的积累。它们在Aβ转基因菌株中也表现出神经保护作用，可以延长寿命和延缓瘫痪。此外，它们显著减少了暴露于UV-A的蠕虫体内ROS的积累，从而表现出抗光老化的潜力。KP和DMF可以激活SKN-1、DAF-16，这从分子对接和qPCR分析中是明显的。在用KP和DMF处理后，DAF-2和DAF-16突变体的寿命没有表现出任何变化，这表明DAF-16介导的途径参与调节抗衰老和神经保护作用。结论：我们的研究结果表明，以DMF为活性成分的KP是一种潜在的治疗衰老和相关疾病的营养品。

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来源期刊

Nutrition and Healthy Aging Agricultural and Biological Sciences-Food Science

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： Nutrition and Healthy Aging is an international forum for research on nutrition as a means of promoting healthy aging. It is particularly concerned with the impact of nutritional interventions on the metabolic and molecular mechanisms which modulate aging and age-associated diseases, including both biological responses on the part of the organism itself and its micro biome. Results emanating from both model organisms and clinical trials will be considered. With regards to the latter, the journal will be rigorous in only accepting for publication well controlled, randomized human intervention trials that conform broadly with the current EFSA and US FDA guidelines for nutritional clinical studies. The journal will publish research articles, short communications, critical reviews and conference summaries, whilst open peer commentaries will be welcomed.