Molecular detection of spa gene among Staphylococcus aureus isolated from mastitis

Iranian Journal of Ichthyology Pub Date : 2021-03-07 DOI:10.22034/IJI.V8I0.589

S. Al-Muhanna, Israa Abdul Ameer Al-Kraety, S. Banoon

引用次数: 0

Abstract

From June to September 2020, among thirty gram-positive bacteria grown with mannitol salt agar, seventeen Staphylococcus aureus isolates were described. Staphylococcus aureus isolates were described in the initial identification based on the colonial morphology, microscopic analysis, and biochemical tests. The final identification was performed using an automated VITEK-2 compact system. The spa genes were detected by the genotyping method using the polymerase chain reaction (PCR) technique. The results showed that the spa gene comprised 19 isolates (63.3%) of S. aureus .

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乳腺炎金黄色葡萄球菌spa基因的分子检测

2020年6月至9月，在用甘露醇盐琼脂培养的30株革兰氏阳性菌中，描述了17株金黄色葡萄球菌分离株。根据菌落形态、显微镜分析和生化测试，对金黄色葡萄球菌分离株进行了初步鉴定。使用自动化VITEK-2紧凑型系统进行最终鉴定。采用聚合酶链式反应（PCR）技术进行spa基因分型。结果表明，spa基因包含19个金黄色葡萄球菌分离株（63.3%）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Ichthyology Agricultural and Biological Sciences-Aquatic Science

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： Iranian Journal of Ichthyology (IJI) is a peer-reviewed journal for publication of high quality papers on any aspect of ichthyology and will be published 4 times a year by the Iranian Society of Ichthyology (http://www.isi-org.ir). The journal welcomes the submission of manuscripts that meets the general criteria of significance and scientific excellence, and will publish original scientific articles in the fields of ichthyology. The editors welcome the submission of original manuscripts including Research Articles, Short communications, and Views & News. Review papers by distinguished authors and Special Issues will also be considered. It is our aim to provide the international scientific community with an efficiently published journal, meeting high scientific and technical standards. Scopes of Iranian Journal of Ichthyology includes: Systematics, taxonomy, morphology, anatomy, biogeography, biodiversity, conservation, ecology and general biology of fishes (reproduction, food and feeding habits, age and growth, population dynamics, etc.). Papers on freshwater, brackish, marine and fossil fishes will be considered.