The Effects of Multiple Power Densities of Carbon Dioxide Laser on Photothermal Damage in Rat Skin Tissue

Q3 Agricultural and Biological Sciences
Zahra Al-Timimi, Zeina J. Tammemi, Muhammad Akram
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Abstract

A CO2 laser produces infrared photons that are largely absorbed by the skin and cause morphological alterations. Twenty-four (Wistar) rats weighing 290-380 g and ranging in age from 8 months to a year were chosen at random and divided into sixteen rats for histological examination and eight rats for tensile testing to determine the extent of injury caused by photothermal damage induced by multiple doses of a CO2 laser. Anesthesia was achieved with intramuscular doses of 10 mg/kg ketamine and 60 mg/kg Xylazine. Two equal 0.5 cm surgical incisions of rat dorsal skin were performed on the left and right sides. One was utilized as a control while the other was subjected to a 10600 nm CO2 laser at various power levels (12.5, 14.1, 15.6, and 17.2) W/cm2. According to the histological analysis, the non-irradiated skin appeared to be flawless, and normal skin layers were observed. The amount of radiation in the irradiated skin samples was closely related to tissue damage. Higher dosages of irradiation resulted in the most severe cellular mutilation. Tissue injury manifested as epidermal obliteration, coagulation, homogeneous hyalinization, and hair loss. The effects of CO2 laser interaction with the skin were explored in-depth in this study. Exposure to the CO2 laser resulted in severe burns and coagulation.  
不同功率密度二氧化碳激光对大鼠皮肤组织光热损伤的影响
二氧化碳激光产生的红外光子大部分被皮肤吸收,并导致形态改变。随机选取体重290 ~ 380 g、年龄8月龄~ 1岁的Wistar大鼠24只,分为16只进行组织学检查,8只进行拉伸试验,测定多剂量CO2激光光热损伤的损伤程度。肌肉注射剂量为10 mg/kg氯胺酮和60 mg/kg噻嗪。取大鼠背部皮肤左右两侧各二个相等的0.5 cm手术切口。其中一组作为对照,而另一组则受到10600 nm CO2激光的照射,激光的功率水平不同(12.5、14.1、15.6和17.2)W/cm2。组织学分析显示,未照射皮肤无瑕疵,皮肤层数正常。辐照皮肤样品中的辐射量与组织损伤密切相关。较高剂量的辐照导致最严重的细胞损伤。组织损伤表现为表皮闭塞、凝固、均匀透明化和脱发。本研究深入探讨了CO2激光与皮肤相互作用的影响。暴露在二氧化碳激光下导致了严重的烧伤和凝血。
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来源期刊
Current Applied Science and Technology
Current Applied Science and Technology Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
1.50
自引率
0.00%
发文量
51
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