MORPHOLOGICAL CHARACTERIZATION AND GENETIC DIVERSITY USING THE SSR TECHNIQUE IN SOME APPLE GENOTYPES

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2022-12-22 DOI:10.33045/fgr.v38.2022.09

Adina Iancu, M. Chivu

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引用次数: 0

Abstract

The scab is a widespread disease throughout the world causing large financial losses in apple production and needs to be controlled also by developing polygenic varieties with resistance to several races of the pathogen Venturia inaequalis by incorporation of two or more functionally different resistance genes. The studies for evidence of interest genes relating to the resistance of apple varieties to apple scab have been conducted using several types of molecular markers (SCAR, SSR, RAPD, ISSR etc.). In this experiment, using six SSR molecular markers (CH02b10, CH05e03, CH02d01, Hi07f01 and Hi07h02), was tracked the reveal of amplified fragments, corresponding to PCR products associated with resistance genes Rvi2, Rvi8, Rvi5 and Rvi11, but also the intraspecific diversity expressed at the molecular level of Romanian apple varieties, some of them having common genitors. The position of the amplified fragments on the agarose gel for the six SSR markers was located on similar values ranges to those published in various specialized papers, the size of the amplified fragments following to be evaluated by the sequencing step and published in a new paper as an addition to the results of this study.

查看原文本刊更多论文

利用SSR技术对苹果部分基因型的形态特征及遗传多样性分析

结痂病是一种在世界范围内广泛传播的疾病，给苹果生产造成了巨大的经济损失，还需要通过结合两种或两种以上功能不同的抗性基因，培育多基因品种，以抵抗几种致病的不均等文图里亚。利用SCAR、SSR、RAPD、ISSR等分子标记，对苹果品种抗苹果赤霉病相关感兴趣基因进行了证据研究。本实验利用6个SSR分子标记(CH02b10、CH05e03、CH02d01、Hi07f01和Hi07h02)，追踪了抗性基因Rvi2、Rvi8、Rvi5和Rvi11相关PCR产物的扩增片段的揭示，以及罗马尼亚苹果品种在分子水平上表达的种内多样性，其中部分品种具有共同的亲本。6个SSR标记的扩增片段在琼脂糖凝胶上的位置与各种专业论文中发表的值范围相似，扩增片段的大小有待测序步骤评估，并作为本研究结果的补充发表在一篇新论文中。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.