Neural stem cells influenced by ultrasound: Frequency and energy density dependencies

Q3 Medicine

Physics in Medicine Pub Date : 2017-12-01 DOI:10.1016/j.phmed.2017.09.001

Anne Schuster , Holger Rabe , Tanja Schwab , Michael Bischof , Christian Degel , Markus Klotz , Karl-Herbert Schäfer

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引用次数: 3

Abstract

Therapeutic ultrasound is a widely used application in clinics. The response to ultrasound is tissue specific and the parameters of ultrasonic treatments are decisive. Here, the responses of primary enteric (ENS) and central nervous system (CNS) neural stem cells were investigated to different frequency – energy density – combination treatments between 510 kHz–3 W s/cm² and 4.36 MHz–25 W s/cm². Responses to ultrasonic treatments were both frequency and energy density dependent. Ultrasound enhanced the expansion and showed enlarged neurospheres for both ENS and CNS neural stem cell cultures. Differentiation was not impaired in regard to neuronal and glial cell number as well as neurite and glial fiber outgrowth. Ultrasound is a promising tool to expand neural stem cells in vitro neither influencing neurogenesis nor gliogenesis by applying specific frequency – energy density – combinations.

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超声对神经干细胞的影响:频率和能量密度依赖性

超声治疗在临床上有着广泛的应用。超声的反应是组织特异性的，超声治疗的参数是决定性的。本研究研究了原代肠(ENS)和中枢神经系统(CNS)神经干细胞对510 kHz-3 W s/cm2和4.36 MHz-25 W s/cm2之间不同频率-能量密度组合处理的反应。对超声治疗的反应与频率和能量密度有关。超声增强了ENS和CNS神经干细胞培养的扩增并显示增大的神经球。在神经元和神经胶质细胞数量以及神经突和神经胶质纤维的生长方面，分化未受到损害。超声是体外扩增神经干细胞的一种很有前途的工具，既不影响神经发生，也不影响胶质瘤的发生，通过应用特定的频率-能量密度组合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physics in Medicine Physics and Astronomy-Instrumentation

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The scope of Physics in Medicine consists of the application of theoretical and practical physics to medicine, physiology and biology. Topics covered are: Physics of Imaging Ultrasonic imaging, Optical imaging, X-ray imaging, Fluorescence Physics of Electromagnetics Neural Engineering, Signal analysis in Medicine, Electromagnetics and the nerve system, Quantum Electronics Physics of Therapy Ultrasonic therapy, Vibrational medicine, Laser Physics Physics of Materials and Mechanics Physics of impact and injuries, Physics of proteins, Metamaterials, Nanoscience and Nanotechnology, Biomedical Materials, Physics of vascular and cerebrovascular diseases, Micromechanics and Micro engineering, Microfluidics in medicine, Mechanics of the human body, Rotary molecular motors, Biological physics, Physics of bio fabrication and regenerative medicine Physics of Instrumentation Engineering of instruments, Physical effects of the application of instruments, Measurement Science and Technology, Physics of micro-labs and bioanalytical sensor devices, Optical instrumentation, Ultrasound instruments Physics of Hearing and Seeing Acoustics and hearing, Physics of hearing aids, Optics and vision, Physics of vision aids Physics of Space Medicine Space physiology, Space medicine related Physics.