Amplification of SERS Signal of Methotrexate Using Beta-Cyclodextrin Modified Silver Nanoparticles

IF 2.5 Q3 CHEMISTRY, PHYSICAL

Colloids and Interfaces Pub Date : 2023-05-26 DOI:10.3390/colloids7020042

N. E. Markina, I. Goryacheva, A. Markin

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引用次数: 0

Abstract

The paper describes the use of native β-cyclodextrin (CD) for the modification of silver nanoparticles (AgNPs) in order to improve the determination of the anticancer drug methotrexate (MTX) using surface-enhanced Raman spectroscopy (SERS). A control experiment with unmodified AgNPs showed that the strong SERS signal of MTX can only be achieved in alkaline media. However, competitive interactions and the strong background signal of human body fluid components significantly challenge MTX determination in real samples. While previous reports propose the use of thorough sample pretreatment (e.g., solid phase extraction), the application of CD-modified AgNPs increases the SERS signal of MTX in neutral media by seven times which enables simplifying the analysis and improving its accuracy by reducing the influence of endogenous components of body fluids. A detailed study of the synthesis conditions (CD concentration and reaction time) and SERS registration conditions (pH, NaCl concentration, dilution of urine samples) was performed to maximize the analytical signal and signal-to-noise ratio. The final assay was tested for MTX determination in artificially spiked samples of real human urine. The results demonstrated that MTX can be determined within the concentration range suitable for therapeutic drug monitoring (20–300 μg mL−1) with satisfactory precision (6–15% RSD), accuracy (95–111% apparent recovery), and limit of detection (0.3 μg mL−1).

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β -环糊精修饰纳米银对甲氨蝶呤SERS信号的放大研究

本文介绍了利用天然β-环糊精（CD）对银纳米粒子（AgNPs）进行修饰，以改进表面增强拉曼光谱（SERS）对抗癌药物甲氨蝶呤（MTX）的测定。用未修饰的AgNPs进行的对照实验表明，MTX的强SERS信号只能在碱性介质中获得。然而，竞争性相互作用和人体体液成分的强背景信号对真实样本中的MTX测定提出了重大挑战。虽然先前的报告建议使用彻底的样品预处理（例如，固相萃取），但CD修饰的AgNPs的应用将MTX在中性介质中的SERS信号增加了7倍，这使得能够简化分析并通过减少体液内源性成分的影响来提高其准确性。对合成条件（CD浓度和反应时间）和SERS登记条件（pH、NaCl浓度、尿样稀释）进行了详细研究，以最大限度地提高分析信号和信噪比。在人工掺入的真实人类尿液样品中测试最终测定MTX。结果表明，MTX可以在适用于治疗药物监测的浓度范围内（20–300μg mL−1）进行测定，具有令人满意的精密度（6–15%的RSD）、准确度（95–111%的表观回收率）和检测限（0.3μg mL–1）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colloids and Interfaces CHEMISTRY, PHYSICAL-

CiteScore

3.90

自引率

4.20%

发文量

审稿时长

10 weeks