Clostridium difficile toxin B-induced apoptosis of colon cancer cells and related mechanisms

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2020-01-31 DOI:10.3760/CMA.J.ISSN.0254-5101.2020.01.010

Xiaolang Huang, Cheng-ji Wang, W. Bao, H. Cai, Qingjie Peng

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Abstract

Objective To analyze the effects of Clostridium difficile toxin B (TcdB) on the proliferation and apoptosis of colon cancer cell line SW480 and the possible mechanisms related to cell apoptosis. Methods SW480 cells were treated with different concentrations of TcdB. Cell proliferation was detected by MTT assay. Cell apoptosis and mitochondrial membrane potential were measured with flow cytometry. Results TcdB significantly inhibited the proliferation of SW480 cells in a time-concentration dependent manner and the inhibition rate reached 46.36% at 48 h. Flow cytometry results showed that TcdB could induce the apoptosis of SW480 cells in a time-concentration dependent manner and a 20.83% apoptosis rate was induced by 800 ng/ml of TcdB at 48 h. Conclusions TcdB could inhibit the proliferation and induce the apoptosis of colon cancer SW480 cells, and the possible mechanisms might be relate to the initiation of mitochondrial apoptosis pathway. Key words: Clostridium difficile toxin B; Colon cancer SW480 cells; Proliferation; Apoptosis

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艰难梭菌毒素B诱导结肠癌癌症细胞凋亡及其相关机制

目的分析艰难梭菌毒素B（TcdB）对结肠癌细胞株SW480增殖和凋亡的影响及其可能的细胞凋亡机制。方法用不同浓度的TcdB处理SW480细胞。MTT法检测细胞增殖。流式细胞仪检测细胞凋亡和线粒体膜电位。结果TcdB对SW480细胞增殖有明显的抑制作用，且呈时间-浓度依赖性，48h抑制率达46.36%。流式细胞术结果显示，TcdB能诱导SW480细胞凋亡，且呈时间-浓度依赖性，在48 h时，800 ng/ml TcdB诱导SW480凋亡率为20.83%。结论TcdB能抑制癌症SW480细胞增殖并诱导其凋亡，其机制可能与线粒体凋亡途径的启动有关。关键词：艰难梭菌毒素B；癌症结肠癌SW480细胞；扩散；细胞凋亡

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.