Elemental Probe-Based CRISPR/Cas12a Biosensing For Sensitive Tobramycin Detection

Abstract

: The precise targeting and excellent specific cleave capability of CRISPR/Cas (clustered regularly inters-paced short palindromic repeats-associated proteins) have gained much research interest. In addition to nucleic acid detection, researchers exploring the potential of this system for detecting various other targets. Herein, we proposed a novel CRISPR/Cas biosensing based Tobramycin (TOB) detection method. Combining with the aptamer recognition and element labeling strategy, highly sensitive and specific detection of TOB is realized. Due to the powerful elemental analysis capabilities of inductively coupled plasma mass spectrometry (ICP-MS) and the excellent signal amplification effect of CRISPR/Cas, the linear concentration range for TOB was 2.8-100 nM in this method, whereas the detection limit was 0.83 nM. The proposed method has been successfully applied in the analysis of actual water samples.