Sensitivity in Callus Tissue of Plectranthus amboinicus (L.) through Mutation Induction with Colchicine

IF 0.6 Q4 AGRONOMY

Agrivita Pub Date : 2022-02-01 DOI:10.17503/agrivita.v44i1.3058

Kunto Wibisono, S. Aisyah, W. Nurcholis, S. Suhesti

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引用次数: 1

Abstract

Mutation induction by colchicine is one method to improve plant genetic diversity (polyploidy). This study aims to 1) obtain growth regulators composition suitable in inducing callus, 2) obtain the LC50 value in callus of P. amboinicus, 3) to determine the effect of colchicine on callus growth, 4) to get shoots (putative mutant) varian, regenerating from indirect organogenesis (callus) in MV1. The study is conducted at the Laboratory of Agricultural Seed Management, Plantation Research, and Development Center from June 2018 to June 2019. The study uses a complete randomized design with four treatments and three replications. Explants use ware the nodes. Callus induction uses MS0 medium with 0.5 ppm 2.4-D, 2 ppm 2.4-D, 0.5 ppm 2.4-D + 1 ppm BAP, and 0.5 ppm 2.4-D + 0.5 ppm TDZ treatment. Mutation induction was performed using colchicine concentration at 0%, 0.02%, 0.04%, and 0.06% treatments. The regeneration media is an MS0 medium. The results show the best combination of plant growth regulators to induce the callus is 0.5 ppm 2.4-D. The LC50 colchicine concentration is at 0.0275%. However, this phenotype is reversible. An additional subculture period is required to move to normal morphology

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秋水仙碱诱变对羊草愈伤组织的敏感性

秋水仙碱诱变是提高植物遗传多样性（多倍体）的一种方法。本研究的目的是：1）获得适合于诱导愈伤组织的生长调节剂组合物；2）获得安氏乳杆菌愈伤组织中的LC50值；3）测定秋水仙碱对愈伤组织生长的影响；4）在MV1中获得由间接器官发生（愈伤组织）再生的芽（推定突变体）变体。该研究于2018年6月至2019年6月在农业种子管理、种植研究与发展中心实验室进行。该研究采用了完全随机设计，共有四种治疗方法和三次重复。外植体使用ware节点。愈伤组织诱导使用MS0培养基，其具有0.5ppm 2.4-D、2ppm 2.4-D、0.5ppm 2.4-D+1ppm BAP和0.5ppm 2.4-D-0.5ppm TDZ处理。使用浓度为0%、0.02%、0.04%和0.06%的秋水仙碱处理进行突变诱导。再生介质为MS0介质。结果表明，诱导愈伤组织的最佳植物生长调节剂组合为0.5ppm 2.4-D。秋水仙碱的LC50浓度为0.0275%。然而，这种表型是可逆的。需要额外的继代培养期才能达到正常形态

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来源期刊

Agrivita AGRONOMY-

CiteScore

2.20

自引率

12.50%

发文量

审稿时长

18 weeks

期刊介绍： The aims of the journal are to publish and disseminate high quality, original research papers and article review in plant science i.e.: -agronomy -horticulture -plant breeding -soil sciences -plant protection -other pertinent field related to plant production