in vivo antibacterial and therapeutic properties of P. ostreatus against Staphylococcus aureus

B. A. Oyekanmi, A. Onifade, I. Osho, O. Ajayi
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Abstract

Objectives: The use of natural products is embraced by a larger percentage of the world population. Most species of fungi including mushrooms produce useful secondary metabolites that stimulate the immune system against infection and diseases. Investigations were conducted to assay the therapeutic potentials of P. ostreatus against pathogenic staph infection.Methods: The methanol extract of P. ostreatus was prepared using the cold extraction method. Thirty Wistar albino rats weighing 82.0 g to 99.2 g were distributed into 6 groups of 5 and inoculated orally with actively growing Staphylococcus aureus suspension. P. ostreatus methanol extract LD > 5000 mg/kg 50 was used to determine the graded doses for the study. Graded doses of the extract 625 mg, 1250 mg, and 2500 mg were administered orally to the experimental animals for seven days.Results: The negative control and 625 mg had skin ulceration while 1250 mg to 2500 mg produced apparently healthy skin. Bacterial count after 7 days post-treatment was significantly high in the negative control and 625 mg dose (32.00 x 104 ± 6.10b; 43.40 x 104 ± 6.20b Cfu/ml) P< 0.05. Haematological and serum biochemical values were not significantly P < 0.05 affected. Pleurotus ostreatus administration at 1250 to 2500 mg produced a statistically low colony count that was comparable with 13.33 mg Ciprofloxacin and placebo.Conclusion: Pleurotus ostreatus at 1250 to 2500 mg was able to produce clinical recovery from S. aureus infection while 625 mg could not. The extract had no deleterious effect on the blood parameters, liver enzymes, and kidney biomarkers.  
平菇对金黄色葡萄球菌的体内抗菌和治疗作用
目标:使用天然产品的人口占世界人口的比例更大。包括蘑菇在内的大多数真菌都会产生有用的次级代谢产物,刺激免疫系统抵抗感染和疾病。研究了平菇对葡萄球菌致病性感染的治疗潜力。方法:采用冷提取法制备平菇甲醇提取物。将30只体重82.0g至99.2g的Wistar白化大鼠分为6组,每组5只,并口服生长活跃的金黄色葡萄球菌悬浮液。平菇甲醇提取物LD>5000mg/kg 50用于确定研究的分级剂量。对实验动物口服625mg、1250mg和2500mg的分级剂量的提取物,持续7天。结果:阴性对照组和625 mg有皮肤溃疡,1250 mg至2500 mg有明显的健康皮肤。治疗后7天,阴性对照组的细菌计数显著较高,625 mg剂量(32.00 x 104±6.10b;43.40 x 104±6.20b Cfu/ml)P<0.05。血液学和血清生化值无显著性差异(P<0.05)。平菇1250至2500 mg给药产生的菌落计数在统计学上较低,与13.33 mg环丙沙星和安慰剂相当。结论:1250~2500mg的平菇对金黄色葡萄球菌感染有一定的临床恢复作用,625mg则不能。提取物对血液参数、肝脏酶和肾脏生物标志物没有有害影响。
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