Seroprevalence and Molecular Detection of Brucella Species among Camels at Aswan governorate

Mahmoud Sahban, Essam I Etokhy, M. A. Abd Al-azeem, W. Younis
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引用次数: 0

Abstract

Brucellosis is considered one of the highly contagious disease in animals and is zoonotic disease for human causing undulant fever. So, the objective of this study is to detect the seroprevalence of brucella among camel and detect the brucella species by PCR. A total of 200 serum samples were collected from camel in Aswan governorate and were subjected for mRBPT, CFT. The seropositive sample of mRBPT and some negative serum samples were subjected for PCR to detect Brucella species. the obtained results in this study show that 6% of serum samples were positive by mRBPT. while only 4 % of serum samples were positive by CFT. 9 out of 12 mRBPT positive serum sample were positive by PCR and the Brucella meltiness were detected in 6 out of 9 serum samples and the Brucella suis were detected in 3 serum samples out of 9 positive PCR serum samples.in conclusion detection of brucellosis in camel causing highly economic losses and causing public health hazards on human being.
阿斯旺省骆驼布鲁氏菌的血清流行率和分子检测
布鲁氏菌病被认为是一种高度传染性的动物疾病,是一种由人类引起的人畜共患疾病。因此,本研究的目的是检测骆驼布鲁氏菌的血清流行率,并通过聚合酶链式反应检测布鲁氏菌种类。共从阿斯旺省的骆驼身上采集了200份血清样本,并进行了mRBPT和CFT。对mRBPT血清阳性标本和部分血清阴性标本进行PCR检测布鲁氏菌种。本研究的结果表明,mRBPT阳性率为6%。而CFT阳性率仅为4%。12份mRBPT阳性血清中有9份经PCR检测呈阳性,9份血清中有6份检出溶性布鲁氏菌,9份PCR阳性血清中3份检出猪布鲁氏菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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审稿时长
16 weeks
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