Elucidating the Dynamic Properties of Globular Protein using Predicted Order Parameters and 15N NMR Relaxation

IF 0.4 Q4 BIOCHEMICAL RESEARCH METHODS
Jong-Jae Yi, Won-Je Kim, J. Rhee, Jongsoo Lim, Bong‐Jin Lee, W. Son
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引用次数: 0

Abstract

Dynamic properties of proteins can present key information on protein-ligand and protein-protein interaction. Despite their usefulness, the properties of protein dynamics have not been obtained easily due to protein stability and short-term measurement. Here, it is shown that combined method for analysis of dynamical properties. It utilizes predicted order parameter and NMR relaxation data such as T 1 , T 2 , and heteronuclear NOE. The suggested method could be used to know the flexibility of protein roughly without precise dynamical parameters such as order parameters through model-free analysis.
利用预测序参量和15N核磁共振弛豫分析球状蛋白的动力学性质
蛋白质的动态特性可以提供蛋白质-配体和蛋白质-蛋白质相互作用的关键信息。尽管它们很有用,但由于蛋白质的稳定性和短期测量,蛋白质动力学的性质并不容易获得。本文提出了一种综合分析动力特性的方法。它利用了预测的序参量和核磁共振弛豫数据,如t1、t2和异核NOE。该方法可以通过无模型分析,在不需要精确的序参数等动力学参数的情况下,粗略地了解蛋白质的柔韧性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of the Korean magnetic resonance society
Journal of the Korean magnetic resonance society BIOCHEMICAL RESEARCH METHODS-
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