Effect of LINC00665 on hepatocellular carcinoma cell proliferation, apoptosis, migration and invasion by targeting miR-379-5p

Q4 Medicine

中华肝胆外科杂志 Pub Date : 2020-03-28 DOI:10.3760/CMA.J.ISSN.1007-8118.2020.03.008

Xiting Zhao, R. Ji

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引用次数: 0

Abstract

Objective To investigate the effect and mechanism of LINC00665 on hepatocellular carcinoma cell proliferation, migration, invasion and apoptosis. Methods From June 2013 to June 2018, 126 liver cancer tissue and adjacent tissue (more than 2 cm from the edge of liver cancer tissue) specimens were collected in the Third People's Hospital of Jinan, 86 male and 40 female were included, aged 25.0-72.0 (48.2±9.9) years. The expression level of LINC00665 in 126 liver cancer tissues and adjacent tissues were detected by qRT-PCR. The survival rate of hepatocellular carcinoma HCC9204 cells was determined by CCK8 assay. The apoptosis of HCC9204 cells was detected by flow cytometry, cell migration and invasion were detected by Transwell assay. And the dual-luciferase reporter assay system was implemented to investigate the correlations between LINC00665 and miR-379-5p. Results Compared with the adjacent tissues group, the expression level of LINC00665 in liver cancer tissues group was increased (1.00±0.10 vs. 1.82±0.18), with statistically significant difference (P 0.05); The level of miR-379-5p in the LINC00665 overexpression group (pcDNA3.1-LINC00665) was decreased [(1.01±0.10) vs (0.37±0.04)]; but was increased in the LINC00665 inhibition group (si-LINC00665)[(0.98±0.10) vs (1.66±0.17)], with statistically significant differences (P<0.05); Decreasing the content of LINC00665 and miR-379-5p, The cell survival rate was increased [(46.53±4.72)% vs. (82.26±8.34)%], the apoptosis rate was decreased (23.51±2.44)% vs. (12.07±1.21)%], and the number of migrating cells and invasive cells were increased [(54±6) vs. (92±9); (48±5) vs. (88±9)] when LINC00665 and miR-379-5p were inhibited the difference were statistically significant (all P<0.05). Conclusions In hepatocellular carcinoma HCC9204, LINC00665 targets the regulation of miR-379-5p expression, thereby regulating the proliferation, migration, invasion, and apoptosis of hepatocellular carcinoma HCC9204, which is a potential molecular target for liver cancer. Key words: Liver neoplasms; MiR-379-5p; Proliferation; Migration; Invasion; Apoptosis

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LINC00665靶向miR-379-5p对肝癌细胞增殖、凋亡、迁移和侵袭的影响

目的探讨LINC00665对肝癌细胞增殖、迁移、侵袭和凋亡的影响及其机制。方法2013年6月至2018年6月，在济南市第三人民医院采集癌症组织及邻近组织126例（距癌症组织边缘2cm以上），男86例，女40例，年龄25.0-72.0（48.2±9.9）岁。用qRT-PCR方法检测了LINC00665在126例癌症组织及癌旁组织中的表达水平。CCK8法测定HCC9204细胞的存活率。流式细胞仪检测HCC9204细胞凋亡，Transwell法检测细胞迁移和侵袭。并采用双荧光素酶报告基因测定系统研究LINC00665与miR-379-5p之间的相关性。结果肝癌症组织中LINC00665的表达水平较癌旁组织明显升高（1.00±0.10与1.82±0.18），差异有统计学意义（P＜0.05）；LINC00665过表达组的miR-379-5p水平降低[（1.01±0.10）vs（0.37±0.04）]；但在LINC00665抑制组（si-LINC00665）中增加[（0.98±0.10）vs（1.66±0.17）]，具有统计学意义（P<0.05）；降低LINC00665和miR-379-5p的含量，细胞存活率增加[（46.53±4.72）%vs.（82.26±8.34）%]，凋亡率降低[（23.51±2.44）%vs..（12.07±1.21）%]，并且当LINC00665和miR-379-5p被抑制时，迁移细胞和侵袭细胞的数量增加[（54±6）对（92±9）；（48±5）对（88±9）]，差异具有统计学意义（均P<0.05），以及肝癌HCC9204的凋亡，HCC9204是癌症的潜在分子靶点。关键词：肝肿瘤；MiR-379-5p；扩散；移民；入侵；细胞凋亡

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来源期刊

中华肝胆外科杂志 Medicine-Gastroenterology

CiteScore

0.20

自引率

0.00%

发文量

7101

期刊介绍： Chinese Journal of Hepatobiliary Surgery is an academic journal organized by the Chinese Medical Association and supervised by the China Association for Science and Technology, founded in 1995. The journal has the following columns: review, hot spotlight, academic thinking, thesis, experimental research, short thesis, case report, synthesis, etc. The journal has been recognized by Beida Journal (Chinese Journal of Humanities and Social Sciences). Chinese Journal of Hepatobiliary Surgery has been included in famous databases such as Peking University Journal (Chinese Journal of Humanities and Social Sciences), CSCD Source Journals of China Science Citation Database (with Extended Version) and so on, and it is one of the national key academic journals under the supervision of China Association for Science and Technology.