Panel of Regulatory miRNAs for Blood Coagulation under Normoxic and Hypoxic Conditions

Q3 Pharmacology, Toxicology and Pharmaceutics
A. A. Hembrom, N. Ghosh, Vinay V. Kumar, I. Garg, L. Ganju, Swati Srivastava
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引用次数: 0

Abstract

Abnormal blood coagulation may lead to venous thromboembolism (VTE), a complex multifactorial disease. Hypoxia (oxygen deprivation) is a major factor disturbing the blood hemostasis and predisposing the body towards coagulation and VTE. Pathophysiology of VTE can be attributed to post-transcriptional gene regulation by microRNAs (miRNAs). The present study identified regulatory miRNAs involved in causing blood coagulation under normoxic and hypoxic conditions. Meta-analysis was performed, following PRISMA guidelines, for identifying miRNAs involved in blood coagulation pathway. Studies evaluating miRNAs from circulating blood as potential biomarkers of VTE were selected. A total of 16 studies met selection criteria and 8 having complete statistical information were selected for analysis. Study of blood coagulation mechanism under hypoxic conditions involved in-silico search within highly cited databases to identify miRNAs commonly regulating genes of hypoxia-inducible factor (HIF) family and coagulation pathway. Further bio-informatics approaches were employed to identify potential biomarker candidates. Meta-analysis revealed a panel of 12 miRNAs; two members of miR-27 family, hsa-miR-27a and hsa-miR-27b; two members of miR-320 family, hsa-miR-320a and hsa-miR-320b, hsa-miR-1233, hsa-miR-134, hsa-miR-424-5p, hsa-miR-221, hsa-miR-28-3p, hsa-miR-136-5p, hsa-miR-374-5p and hsa-miR-338-5p involved in blood coagulation under normoxic conditions. Besides these, present in-silico analysis identified a set of 5 miRNAs including hsa-miR-4667-5p, hsa-miR-6815-3, hsa-miR-4433a-3p, hsa-miR-6735-5p and hsa-miR-6777-3p which predominantly regulate genes that facilitate both coagulation and response to hypoxic stress. The present study generated a panel of regulatory miRNAs potentially involved in the process of blood coagulation under both normoxic and hypoxic conditions, which may serve as putative epigenetic biomarkers for coagulation.
常氧和低氧条件下血液凝固的调节mirna组
异常凝血可能导致静脉血栓栓塞症(VTE),这是一种复杂的多因素疾病。缺氧(缺氧)是干扰血液止血和使身体倾向于凝血和VTE的主要因素。VTE的病理生理学可归因于微小RNA(miRNA)的转录后基因调控。本研究确定了在常氧和缺氧条件下引起凝血的调节性miRNA。根据PRISMA指南进行荟萃分析,以鉴定参与凝血途径的miRNA。选择了评估循环血液中miRNA作为VTE潜在生物标志物的研究。共有16项研究符合选择标准,8项具有完整统计信息的研究被选择进行分析。缺氧条件下凝血机制的研究涉及在高度引用的数据库中进行计算机搜索,以确定通常调节缺氧诱导因子(HIF)家族基因和凝血途径的miRNA。进一步的生物信息学方法被用于识别潜在的生物标志物候选者。荟萃分析显示了一组12个miRNA;miR-27家族的两个成员,hsa-miR-27a和hsa-miR27b;miR-320家族的两个成员,hsa-miR-320a和hsa-miR.320b、hsa-miR-1233、hsa-iR-134、hsa-miR-424-5p、hsa--miR-221、hsa-micro-28-3p、hsa-miR-136-5p、hsa miR-374-5p和hsa-micro-338-5p,参与常氧条件下的血液凝固。除此之外,目前的计算机分析鉴定了一组5种miRNA,包括hsa-miR-4667-5p、hsa-miR-6815-3、hsa-iR-4433a-3p、hsa-miR-6735-5p和hsa-miR-6777-3p,它们主要调节促进凝血和对缺氧应激反应的基因。本研究产生了一组可能参与常氧和缺氧条件下凝血过程的调节性miRNA,这些miRNA可能作为凝血的假定表观遗传学生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Defence Life Science Journal
Defence Life Science Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
0.80
自引率
0.00%
发文量
26
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