Study of Phosphate Solubilizing Fluorescent Pseudomonas Recovered from Rhizosphere and Endorhizosphere of Aloe barbadensis (L.)

IF 2.2 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Geomicrobiology Journal Pub Date : 2023-02-01 DOI:10.1080/01490451.2023.2171165

A. Rai, N. Sharma, V. Singh, B. S. Dwivedi, J. Singh, P. Rai

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Abstract

Abstract Phosphorus (P) is a key nutrient required by plants for their growth and development. A large part of applied phosphatic fertilizer becomes unavailable due to its fixation in soil. In rhizosphere, fluorescent Pseudomonads are instrumental in phosphate solubilization. We isolated Pseudomonads from the rhizosphere of Aloe barbadensis (L.), and screened them for their plant growth promoting ability such as - solubilization of phosphate, auxin production and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. A total of 307 fluorescent Pseudomonas were isolated and screened, of which, 24 isolates showed a strong phosphate solubilizing ability, ranging from 69.71 to 236.73 µg ml−1 . The Indole acetic acid (IAA) production by all 24 isolates varied from 28.44 to 151.99 µg ml−1. However, 17 isolates produced ACC-deaminase ranging from 8.19 to 10.27 µmol mg−1 h−1. Analyses of 16S rRNA gene and Pseudomonas-specific genes grouped the isolates in seven different species of fluorescent Pseudomonas. Genotypic analysis based on 16S rDNA-RFLP using restriction enzymes (HaeIII, AluI and MspI) and BOX-PCR generated unique genotype specific pattern. The results suggested that Pseudomonas diversity has no association with microenvironments and the sampling site of the plants. 16S rRNA gene-based diversity indices revealed great diversity among the fluorescent Pseudomonas recovered from the rhizospheric regions of the Aloe. Due to the above plant growth-promoting traits, fluorescent Pseudomonas can be exploited as bio-inoculants for crops, with minimal damage to the environment. GRAPHICAL ABSTRACT

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芦荟根际和内层溶磷荧光假单胞菌的研究

摘要磷(P)是植物生长发育所需的重要营养物质。施用的磷肥有很大一部分由于在土壤中的固结而无法使用。在根际，荧光假单胞菌对磷酸盐的增溶有重要作用。从芦荟根际分离得到假单胞菌，并对其进行了促磷酸、促生长素和促1-氨基环丙烷-1-羧酸脱氨酶活性的筛选。共分离筛选到307株荧光假单胞菌，其中24株具有较强的磷酸盐增溶能力，范围为69.71 ~ 236.73µg ml−1。24株菌株的吲哚乙酸(IAA)产量在28.44 ~ 151.99µg ml−1之间。然而，17株分离株产生的acc脱氨酶范围为8.19 ~ 10.27µmol mg−1 h−1。16S rRNA基因和假单胞菌特异性基因对7种不同的荧光假单胞菌进行了分类。利用限制性内切酶(HaeIII、AluI和MspI)和BOX-PCR对16S rDNA-RFLP进行基因型分析，产生独特的基因型特异性模式。结果表明，假单胞菌多样性与植物微环境和采样地点无关。基于16S rRNA基因的多样性指数显示，芦荟根际区域的荧光假单胞菌具有很强的多样性。由于上述植物生长促进特性，荧光假单胞菌可以作为作物的生物接种剂，对环境的破坏最小。图形抽象

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来源期刊

Geomicrobiology Journal 环境科学-地球科学综合

CiteScore

4.80

自引率

8.70%

发文量

审稿时长

3.3 months

期刊介绍： Geomicrobiology Journal is a unified vehicle for research and review articles in geomicrobiology and microbial biogeochemistry. One or two special issues devoted to specific geomicrobiological topics are published each year. General articles deal with microbial transformations of geologically important minerals and elements, including those that occur in marine and freshwater environments, soils, mineral deposits and rock formations, and the environmental biogeochemical impact of these transformations. In this context, the functions of Bacteria and Archaea, yeasts, filamentous fungi, micro-algae, protists, and their viruses as geochemical agents are examined. Articles may stress the nature of specific geologically important microorganisms and their activities, or the environmental and geological consequences of geomicrobiological activity. The Journal covers an array of topics such as: microbial weathering; microbial roles in the formation and degradation of specific minerals; mineralization of organic matter; petroleum microbiology; subsurface microbiology; biofilm form and function, and other interfacial phenomena of geological importance; biogeochemical cycling of elements; isotopic fractionation; paleomicrobiology. Applied topics such as bioleaching microbiology, geomicrobiological prospecting, and groundwater pollution microbiology are addressed. New methods and techniques applied in geomicrobiological studies are also considered.