Effects of different types of gelling agents on in vitro organogenesis and some physicochemical properties of date palm buds, Showathy cv.

Pub Date : 2021-03-01 DOI:10.2478/foecol-2021-0012

Ahmed Madi Waheed Al-Mayahi, Abdulminam Hussian Ali

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引用次数: 11

Abstract

Abstract Some obstacles are associated with in vitro propagation of date palm, such as explant tissue browning, slow callus growth and development, low organogenesis and multiplication efficiency, and frequent tissue vitrification. This investigation studied the effect of five types of gelling agents (Danish Agar, Cero Agar Type 8952, Chile Agar, Gerlite Food Grade, and Agar-Agar.) on in vitro regeneration and bud multiplication of Phoenix dactylifera L. cv. Showathy. The results showed that the highest percentages of callus producing buds and average bud formation (77.78%, 11.5 buds, and 72.23%, 10.9 buds) were obtained in response to 7 g l–1 Danish Agar and Cero Agar Type 8952, respectively. A decrease in browning percentage was observed in tissues cultured in the medium gelled with Danish Agar. Observations showed that Danish Agar and Cero Agar Type 8952 eliminated also shoot vitrification. Compared with other treatments, the total amount of phenolic compounds was significantly reduced to 0.79 and 0.82 mg GAE/g in buds cultured in the media gelled with Danish Agar and Cero Agar Type 8952, respectively. The macronutrient phosphor, calcium, sodium, and micronutrient boron and copper significantly increased in the in vitro shoots regenerated on the media gelled with Danish Agar and Cero Agar Type 8952.

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不同类型胶凝剂对枣椰树芽离体器官发生及部分理化性质的影响。

摘要枣椰树离体繁殖存在外植体组织褐变、愈伤组织生长发育缓慢、器官发生和增殖效率低、组织玻璃化频繁等障碍。研究了5种胶凝剂(丹麦琼脂、Cero琼脂8952型、智利琼脂、Gerlite食品级和Agar-Agar.)对凤仙花离体再生和芽增殖的影响。Showathy。结果表明，7 g l-1丹麦琼脂和8952型Cero琼脂的愈伤组织出芽率和平均出芽率最高，分别为77.78%、11.5芽和72.23%、10.9芽。在用丹麦琼脂凝胶化的培养基中培养的组织中，观察到褐变百分比的下降。观察表明，丹麦琼脂和8952型Cero琼脂也消除了玻璃化。与其他处理相比，用Danish Agar和Cero Agar Type 8952凝胶培养基培养的芽中酚类化合物的总量分别显著降低至0.79和0.82 mg GAE/g。在丹麦琼脂和8952型Cero琼脂胶凝培养基上再生的离体芽中常量元素磷、钙、钠和微量元素硼、铜显著增加。

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