Cell-in-cell phenomenon in urinary sediment: a case report

IF 3.8 3区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Biochemia Medica Pub Date : 2022-04-15 DOI:10.11613/BM.2022.020801

Carlos Martínez-Figueroa, K. Cortés-Sarabia, J. Poloni, Enrique Alejandro Molina-Avilez, L. Palaoro, A. Vences-Velázquez

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Abstract

The internalization of apoptotic cells by non-phagocytic cells has been observed in different tissues and could be an important mechanism for the elimination of dying cells. Here, we describe a probable event of phagocytosis of apoptotic cells mediated by urothelial cells in urinary sediment. A 90-years-old male patient was admitted unconscious to the hospital, visible signs included: pale skin and dry mucous membranes, presumptively diagnosed as dehydration. Blood test revealed anaemia (haemoglobin 130 g/L) and hyperglycaemia (glucose 7.8 mmol/L), urinalysis showed a picture of urinary tract infection (leukocyturia and bacteriuria). The microscopic analysis of urinary sediment revealed the presence of urothelial cells and leukocytes internalized in urothelial cells. Anti-CD68 (membrane marker of macrophages) was tested by immunocytochemistry and a negative result was observed. Based on the findings phagocytosis of apoptotic cells mediated by urothelial cells was identified. This phenomenon can be observed in urinary sediment and should not be confused with a neoplastic process since it is a physiological event of cell elimination.

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尿沉渣中细胞间现象1例报告

在不同的组织中已经观察到非吞噬细胞对凋亡细胞的内化，这可能是消除死亡细胞的重要机制。在此，我们描述了由尿沉渣中的尿路上皮细胞介导的凋亡细胞吞噬作用的可能事件。一名90岁的男性患者昏迷入院，可见症状包括：皮肤苍白、粘膜干燥，推定诊断为脱水。血液检查显示贫血（血红蛋白130 g/L）和高血糖（葡萄糖7.8 mmol/L），尿液分析显示尿路感染（白细胞尿和细菌尿）。尿沉渣的显微镜分析显示存在尿路上皮细胞和内化于尿路上皮中的白细胞。用免疫细胞化学方法检测抗CD68（巨噬细胞膜标记物），结果为阴性。基于这些发现，鉴定了由尿路上皮细胞介导的凋亡细胞的吞噬作用。这种现象可以在尿沉渣中观察到，不应与肿瘤过程混淆，因为它是细胞消除的生理事件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemia Medica 医学-医学实验技术

CiteScore

5.50

自引率

3.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biochemia Medica is the official peer-reviewed journal of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Journal provides a wide coverage of research in all aspects of clinical chemistry and laboratory medicine. Following categories fit into the scope of the Journal: general clinical chemistry, haematology and haemostasis, molecular diagnostics and endocrinology. Development, validation and verification of analytical techniques and methods applicable to clinical chemistry and laboratory medicine are welcome as well as studies dealing with laboratory organization, automation and quality control. Journal publishes on a regular basis educative preanalytical case reports (Preanalytical mysteries), articles dealing with applied biostatistics (Lessons in biostatistics) and research integrity (Research integrity corner).