Role of basic fibroblast growth factor in epidermal stem cells differentiating into neural stem cells in rats

中华创伤杂志 Pub Date : 2019-07-15 DOI:10.3760/CMA.J.ISSN.1001-8050.2019.07.006

Zheng-wei Xu, D. Hao, Liang Dong, Tuan-jiang Liu, Hua Guo, B. He

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Abstract

Objective To investigate the effect of basic fibroblast growth factor (bFGF) on the differentiation of epidermal stem cells(ESCs) into nerve cells in rats. Methods The epidermal basal layer tissue of newborn SD rats (1-3 days) were isolated and obtained. ESCs were digested and isolated by rapid attachment to a substrate, and the morphology of ESCs was observed under an inverted microscope. ESCs were cultured with Keratinocytes serum-free medium (K-SFM). The ESCs were grouped and treated according to the density including Group A: 0.1×107/ml, Group B: 0.3×107/ml, Group C: 0.5×107/ml, Group D: 0.1×106/ml, and each group was added bFGF (20 ng/ml). The changes of cell morphology were observed and counted for seven days. The changes of cell markers Nestin and NSE were detected by immunohistochemistry. Results The ESCs of SD rat were isolated successfully. After bFGF induction, the numbers of cells with morphological changes in Groups A and B were larger than those in other two groups in the first 6 days (P<0.05), and the number in Group A was the largest on the seventh day (P<0.05). On the third day, Group C had the largest number of cells with changes based on the comparison within the group. There was no change in cell morphology in Group D. Immunohistochemistry showed positive Nestin and NSE. Conclusion The bFGF helps induce the differentiation of ESCs into nerve cells, which is associated with the cell density. Key words: Fibroblast growth factors; Stem cells; Cell differentiation; Nerve cells

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碱性成纤维细胞生长因子在大鼠表皮干细胞向神经干细胞分化中的作用

目的探讨碱性成纤维细胞生长因子（bFGF）对大鼠表皮干细胞向神经细胞分化的影响。方法分离和获得新生SD大鼠（1-3天）的表皮基底层组织。通过快速附着于底物来消化和分离ESCs，并在倒置显微镜下观察ESCs的形态。ESCs用角质形成细胞无血清培养基（K-SFM）培养。ESCs按密度分组处理，包括A组：0.1×107/ml，B组：0.3×107/ml、C组：0.5×107/ml和D组：0.1±106/ml，每组加入bFGF（20ng/ml）。观察细胞形态的变化并计数7天。免疫组化法检测细胞标志物Nestin和NSE的变化。结果成功分离出SD大鼠ESCs。bFGF诱导后，A组和B组在前6天出现形态变化的细胞数均大于其他两组（P<0.05），A组在第7天出现形态改变的细胞数最多（P<0.05）。D组细胞形态无明显变化。免疫组化显示Nestin和NSE阳性。结论bFGF有助于ESCs向神经细胞分化，这与细胞密度有关。关键词：成纤维细胞生长因子；干细胞；细胞分化；神经细胞

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来源期刊

中华创伤杂志

自引率

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发文量

11327

期刊介绍： Chinese Journal of Trauma (International Standard Serial Publication Number: ISSN 1001-8050, Domestic Uniform Serial Publication Number: CN 50-1098/R) was founded in September 1985, which is the only high-level medical professional academic journal that can comprehensively and systematically reflect the achievements and development trends of China's traumatology medicine, and has a wide academic influence in China's traumatology medicine community. It has a wide range of academic influence in China's trauma medicine. Chinese Journal of Trauma is a source journal of China Science and Technology Paper Statistics, a source journal of China Science Citation Database (CSCD), a core journal of China Comprehensive Medicine and Health Care, a source journal of China Academic Journals Comprehensive Evaluation Database (CAJCED), a full-text journal of China Journal Full-text Database (CJFD), a core academic journal of China Center for Scientific Evaluation (RCCSE), a core academic journal of China Traumatology and Traumatology Center (CTC), a core academic journal of China Traumatology Center (RCCSE). RCCSE) core academic journals; Chinese Biomedical Journal Database (CMCC), Chinese Biomedical Journal Citation Database (CBJCED), China Journal Network (CJN), China Academic Journals (CD-ROM), Chinese Academic Journals Abstracts (Chinese Edition), Chemical Abstracts of the United States (CA), Index Copernicus of Poland (IC), and Japan Institute of Science and Technology Database (JICST), World Health Organization Western Pacific Region Medical Search (WPRIM) and Russian Journal of Abstracts (ΡЖ) included journals.