Isolation, Purification and Complex Formation of Nicotine Alkaloid

S. Mahmood, S. Jameel, Zaheer Ahmad
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引用次数: 2

Abstract

Nicotiana tabacum is rich with medicinal importance. The aim of the study is to isolate, purify and compare nicotine (3-[1-Methylpyrolidine2-yl] pyridine) from different brands of cigarettes. Isolation is carried out by liquid-liquid extraction using ether as solvent. Crude nicotine samples are further purified with SPE (solid phase extraction) method. Nicotine crystals are complexed with zinc chloride (ZnCl2). Fine and pure crystals of metal complexes are obtained with Nic-C and Nic-D. However, no complex formation is synthesized with Nic-A and Nic-B due to impurities in the sample which is confirmed by thin layer chromatography. Antibacterial activity of the samples is carried out against different strains of bacteria which showed positive results for Nic-C and Nic-D. The outcomes of the study reveal that Zinc-Nicotine complexes can be used as therapeutic agents and anti-sickness agents in sickle cell disease.
烟碱生物碱的分离纯化及复合物的形成
烟草具有丰富的药用价值。本研究的目的是分离、纯化和比较不同品牌香烟中的尼古丁(3-[1-甲基吡啶2-基]吡啶)。以乙醚为溶剂,采用液-液萃取法进行分离。采用固相萃取法对粗尼古丁样品进行进一步纯化。尼古丁晶体与氯化锌(ZnCl2)络合。用镍- c和镍- d制备了精细、纯净的金属配合物晶体。然而,由于样品中杂质的存在,镍- a和镍- b没有合成配合物,这一点经薄层色谱证实。对不同菌种进行了抑菌活性测定,结果显示对nicc和nicd的抑菌活性呈阳性。本研究结果表明,锌-尼古丁复合物可作为镰状细胞病的治疗药物和抗病药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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