Effect of porous tantalum on the biological response of human peripheral mononuclear cells exposed to Porphyromonas gingivalis

Q1 Medicine

Journal of Investigative and Clinical Dentistry Pub Date : 2019-09-27 DOI:10.1111/jicd.12472

Eduardo Frois Temponi, Paulo Eduardo Alencar Souza, Giovanna Ribeiro Souto, Luísa Mourão Dias Magalhães, Walderez Ornelas Dutra, Kenneth John Gollob, Tarcília Aparecida Silva, Rodrigo Villamarim Soares

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引用次数: 1

Abstract

Aim

To evaluate biological behavior of human peripheral mononuclear cells (PBMC) in contact with porous tantalum (PT) and Porphyromonas gingivalis (Pg).

Methods

Pg was incubated for 8 hours. The groups formed were: PBMC (control), PBMC + PT, PBMC + Pg and PBMC + PT + Pg. Cell viability was evaluated using MTT assay. The morphology and adhesion of PBMC to PT was evaluated using scanning electron microscopy. Expression of interleukin (IL)-10, transforming growth factor (TGF)-β, matrix metallopeptidase (MMP)-9 and receptor activator of nuclear factor-κΒ ligand (RANKL) was determined by enzyme-linked immunosorbent assay.

Results

MTT assay revealed that PT did not interfere in the mitochondrial activity of PBMC (P > .05). Scanning electron microscopy showed the adherence of PBMC to PT. IL-10 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg. TGF-β levels in PBMC + PT were higher than PBMC and PBMC + Pg. MMP-9 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg and PBMC + PT + Pg. RANKL levels in PBMC + PT were lower than in PBMC.

Conclusion

PT did not affect PBMC viability, allowed cell adhesion, reduced expression of RANKL and enhanced TGF-β in comparison with the control group.

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多孔钽对暴露于牙龈卟啉单胞菌的人外周血单核细胞生物学反应的影响。

目的探讨人外周血单核细胞(PBMC)与多孔钽(PT)和牙龈卟啉单胞菌(Pg)接触后的生物学行为。方法培养8 h。各组分别为PBMC(对照)、PBMC + PT、PBMC + Pg和PBMC + PT + Pg，采用MTT法测定细胞活力。扫描电镜观察PBMC与PT的形貌及粘附性。采用酶联免疫吸附法检测白细胞介素(IL)-10、转化生长因子(TGF)-β、基质金属肽酶(MMP)-9、核因子受体激活剂-κΒ配体(RANKL)的表达。结果smtt检测显示，PT对PBMC线粒体活性无明显影响(P < 0.05)。扫描电镜显示PBMC与PT黏附，PBMC + PT中IL-10水平与PBMC相似，低于PBMC + Pg, TGF-β水平高于PBMC和PBMC + Pg, MMP-9水平与PBMC相似，低于PBMC + Pg和PBMC + PT + Pg, RANKL水平低于PBMC。结论与对照组相比，pt不影响PBMC的活力，使细胞粘附，降低RANKL的表达，增强TGF-β。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Investigative and Clinical Dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： Journal of Investigative and Clinical Dentistry (JICD) aims to publish quality, peer reviewed original research and topical reviews on all aspects of investigative and clinical dentistry and craniofacial research, including molecular studies related to oral health and disease. Although international in outlook, the Editor especially encourages papers from the Asia Pacific. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports through an international journal, which will be available free online until 2012. Its scope, therefore, is broad, inclusive and international, but with a particular focus on Asia Pacific. The Editor welcomes manuscripts in the following key thematic areas in oral and maxillofacial sciences: Community Dentistry and Oral Epidemiology, Conservative Dentistry, Dental Biomaterials, Dental Pedagogy, Endodontics and Traumatology, Implant Dentistry, Oral Biosciences, Oral and Maxillofacial Surgery, Oral Medicine, Oral Pathology and Oral Microbiology, Orthodontics, Oral Radiology, Oral Rehabilitation, Paedodontics, Periodontology and Periodontal Medicine.