EVIDENCE OF A FUNCTIONAL RENIN-ANGIOTENSIN SYSTEM IN THE CHANNEL CATFISH (ICTALURUS PUNCTATUS)

Q4 Agricultural and Biological Sciences

The Texas Journal of Science Pub Date : 2018-10-01 DOI:10.32011/TXJSCI_70_1_ARTICLE2

J. Hunt, K. Davis, M. Sanderford

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Abstract

Salination of freshwater (FW) bodies has the potential to affect homeostatic regulation of osmotic and volume balance in FW organisms. The renin-angiotensin system (RAS) plays an important role in volume balance by maintaining blood pressure in marine and seawater acclimated euryhaline fish, but little is known about the RAS in FW adapted fish. The purpose of the present study was to first determine if the FW channel catfish (Ictalurus punctatus), demonstrates evidence of a functional RAS. Channel catfish (n = 6) were implanted with a catheter in the dorsal aorta to measure dorsal aortic pressure (PDA) and infuse drugs. Infusion of [Asn1,Val5,Asn9]-angiotensin I (ANGI) at 100, 400, and 1000 ng/kg significantly increased PDA in a dose dependent manner (P < 0.05). Pretreatment with 2 mg/kg of the angiotensin converting enzyme inhibitor, Captopril (CAP), essentially eliminated the pressor response to the highest dose of ANGI (P < 0.05). Finally, infusion of 400 ng/kg [Asn1,Val5]-angiotensin II (ANGII) significantly increased PDA from baseline (P < 0.05). The results suggest that channel catfish appear to have an operational RAS and may serve as a suitable model in which to study the role of ANGII in blood pressure regulation in FW adapted fish.

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斑点叉尾鱼肾素-血管紧张素系统功能的证据

淡水(FW)体的盐化有可能影响FW生物体内渗透和体积平衡的稳态调节。肾素-血管紧张素系统(RAS)在海洋和海水适应的广盐鱼中通过维持血压在体积平衡中起重要作用，但对适应海水的广盐鱼的RAS知之甚少。本研究的目的是首先确定FW通道鲶鱼(Ictalurus punctatus)是否具有功能性RAS的证据。6只通道鲶鱼在背主动脉内植入导管，测量背主动脉压(PDA)并输注药物。100、400和1000 ng/kg输注[Asn1,Val5,Asn9]-血管紧张素I (ANGI)显著增加PDA，且呈剂量依赖性(P < 0.05)。2 mg/kg血管紧张素转换酶抑制剂卡托普利(Captopril, CAP)预处理基本上消除了对最高剂量ANGI的升压反应(P < 0.05)。最后，输注400 ng/kg [Asn1,Val5]-血管紧张素II (ANGII)较基线显著增加PDA (P < 0.05)。结果表明，通道鲶鱼似乎具有可操作的RAS，可以作为研究ANGII在FW适应鱼血压调节中的作用的合适模型。

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来源期刊

The Texas Journal of Science Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

0.50

自引率

0.00%

发文量

期刊介绍： Scholarly manuscripts reporting original research results in any field of science or technology, including science education, will be considered for publication in The Texas Journal of Science. Prior to acceptance, each manuscript will be reviewed by both knowledgeable peers and the editorial staff. Authors are encouraged to suggest the names and addresses of two potential reviewers to the Manuscript Editor at the time of submission of their manuscript.