Electrochemical Evaluation of the Catalytic Effect of Natural Phosphate on the Oxidation of Aascorbic Acid: Analytical Application in Human Blood

IF 1.4 Q3 CHEMISTRY, MULTIDISCIPLINARY

Physical Chemistry Research Pub Date : 2021-03-01 DOI:10.22036/PCR.2020.245564.1820

Hayat E.L.Ouafy, T. E. Ouafy, M. Oubenali, M. Mbarki, M. Echajia

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引用次数: 1

Abstract

This work describes the electroanalytical activity of the natural phosphate modified carbon paste electrode (CPE-NP) for ascorbic acid (AA) oxidation, where its electrochemical response is compared to the carbon paste electrode (CPE). The voltammetric behavior of AA was studied when an anodic peak has appeared at 0.35 V (vs. Ag/AgCl/3 M KCl) in the Britton Robinson buffer solution (pH 6.0). This peak was obtained from the irreversible oxidation of AA at CPE-NP. The electro-catalytic influence was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). To give electroanalytical detection to oxidizing the AA on the CPE-NP, the differential pulse voltammograms have been used in the potential range from -0.5 V to 1.5 V vs. Ag/AgCl/(sat. KCl). Besides, CPE-NP can be employed successfully to ameliorate the detection of AA at very feeble concentration with good sensitivity. The calibration range has been from 1.0 × 10-6 to 1.0 ×10-3 mol L-1. The calculated limit of detection (LD) is 1.44 × 10-9 mol L-1 when the relative standard deviation (RSD) at 1.0× 10-5 mol L1 AA concentration was 3.29% for nine repetitions. Afterward, the studied method has been applied to detect AA in human blood with satisfactory results.

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天然磷酸盐对抗坏血酸氧化催化作用的电化学评价:在人体血液中的分析应用

本研究描述了天然磷酸盐修饰碳糊电极(CPE- np)对抗坏血酸(AA)氧化的电分析活性，并将其电化学响应与碳糊电极(CPE)进行了比较。研究了AA在pH为6.0的briton Robinson缓冲溶液中，在0.35 V (vs. Ag/AgCl/3 M KCl)出现阳极峰时的伏安行为。这个峰是由AA在CPE-NP上的不可逆氧化得到的。采用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了电催化的影响。为了对CPE-NP上的AA氧化进行电分析检测，在-0.5 V ~ 1.5 V vs. Ag/AgCl/(sat)电位范围内使用差分脉冲伏安图。氯化钾)。此外，CPE-NP可以成功地改善极弱浓度下对AA的检测，并且具有良好的灵敏度。校准范围为1.0 × 10-6至1.0 ×10-3 mol L-1。在1.0× 10-5 mol L1 AA浓度下，计算检出限(LD)为1.44 × 10-9 mol L-1，相对标准偏差(RSD)为3.29%。将该方法应用于人体血液中AA的检测，结果令人满意。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physical Chemistry Research CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

2.70

自引率

8.30%

发文量

期刊介绍： The motivation for this new journal is the tremendous increasing of useful articles in the field of Physical Chemistry and the related subjects in recent years, and the need of communication between Physical Chemists, Physicists and Biophysicists. We attempt to establish this fruitful communication and quick publication. High quality original papers in English dealing with experimental, theoretical and applied research related to physics and chemistry are welcomed. This journal accepts your report for publication as a regular article, review, and Letter. Review articles discussing specific areas of physical chemistry of current chemical or physical importance are also published. Subjects of Interest: Thermodynamics, Statistical Mechanics, Statistical Thermodynamics, Molecular Spectroscopy, Quantum Chemistry, Computational Chemistry, Physical Chemistry of Life Sciences, Surface Chemistry, Catalysis, Physical Chemistry of Electrochemistry, Kinetics, Nanochemistry and Nanophysics, Liquid Crystals, Ionic Liquid, Photochemistry, Experimental article of Physical chemistry. Mathematical Chemistry.