First report on ALS herbicide resistance in barnyardgrass (Echinochloa crus-galli) from rice fields of India

IF 1.3 3区农林科学 Q3 AGRONOMY

Weed Technology Pub Date : 2023-04-13 DOI:10.1017/wet.2023.24

V. Choudhary, S. S. Reddy, Subhash K. Mishra, Y. Gharde, Sunil Kumar, M. Yadav, S. Barik, P.K. Singh

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Abstract

Abstract Bispyribac-sodium, a herbicide that inhibits acetolactate synthase (ALS), is frequently used in rice fields in India to control weeds, including the most common noxious weed, barnyardgrass. However, rice growers have recently reported reduced control of barnyardgrass with bispyribac-sodium. Hence, a large-scale survey was carried out to assess bispyribac-sodium resistance in Chhattisgarh and Kerala, two rice-growing states. Open-field pot experiments were conducted for 2 yr to confirm resistance to bispyribac-sodium. Of the 37 biotypes tested, 30% (11) survived the recommended label rate of bispyribac-sodium (25 g ai ha–1). The effective rate of bispyribac-sodium required to achieve 50% control (ED50) of putative resistant biotypes ranged from 18 to 41 g ha–1, whereas it was about 10 g ha–1 for susceptible biotypes. This suggests that putative biotypes were two to four times more resistant to bispyribac-sodium. At 6 d after herbicide application, an in vitro enzyme assay demonstrated higher ALS enzyme activity in putative resistant biotypes (66% to 75%) compared with susceptible biotypes (48% to 52%). This indicates the presence of an insensitive ALS enzyme in those biotypes and a target site mutation as a possible mechanism for resistance. Whole-plant bioassays also suggested that the resistance problem is more widespread in Chhattisgarh than in Kerala. This study confirmed the first case of evolved resistance in barnyardgrass to bispyribac-sodium in rice fields of India. Nomenclature: Bispyribac-sodium; barnyardgrass; Echinochloa crus-galli (L.) P. Beauv.; rice; Oryza sativa L.

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印度稻田Barnyardrass（Echinochloa crus galli）对ALS除草剂耐药性的首次报告

摘要Bispyribac钠是一种抑制乙酰乳酸合成酶（ALS）的除草剂，在印度的稻田中经常用于控制杂草，包括最常见的有毒杂草Barnyardrass。然而，水稻种植者最近报告说，双嘧菌钠减少了对Barnyardrass的控制。因此，在恰蒂斯加尔邦和喀拉拉邦这两个水稻种植州进行了一项大规模调查，以评估双嘧菌对钠的抗性。进行了为期2年的露天盆栽试验，以确认对双嘧菌钠的抗性。在测试的37种生物型中，30%（11）的生物型在双嘧菌钠的推荐标记率（25 g ai ha–1）下存活。实现假定抗性生物型50%控制（ED50）所需的双嘧菌酯钠的有效率范围为18至41 g ha–1，而敏感生物型的有效率约为10 g ha–1。这表明，假定的生物型对双嘧菌钠的抗性高出两到四倍。除草剂施用后6天，体外酶测定显示，与易感生物型（48%至52%）相比，推定抗性生物型（66%至75%）的ALS酶活性更高。这表明在这些生物型中存在不敏感的ALS酶，并且靶位点突变可能是抗性的机制。全植物生物测定还表明，抗性问题在恰蒂斯加尔邦比在喀拉拉邦更为普遍。这项研究证实了印度稻田中首次出现Barnyardrass对双嘧菌钠的进化抗性。命名法：双嘧菌酯钠；barnyardgrass；鹅掌棘球蚴（Echinochloa crus galli（L.）P.Beauv.）。；大米水稻。

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来源期刊

Weed Technology 农林科学-农艺学

CiteScore

2.90

自引率

21.40%

发文量

审稿时长

12-24 weeks

期刊介绍： Weed Technology publishes original research and scholarship in the form of peer-reviewed articles focused on understanding how weeds are managed. The journal focuses on: - Applied aspects concerning the management of weeds in agricultural systems - Herbicides used to manage undesired vegetation, weed biology and control - Weed/crop management systems - Reports of new weed problems -New technologies for weed management and special articles emphasizing technology transfer to improve weed control -Articles dealing with plant growth regulators and management of undesired plant growth may also be accepted, provided there is clear relevance to weed science technology, e.g., turfgrass or woody plant management along rights-of-way, vegetation management in forest, aquatic, or other non-crop situations. -Surveys, education, and extension topics related to weeds will also be considered