Moderate Intensity Exercise Effect Reproductive Cycle on High Calories Diet in Mice (Mus Musculus)

Abstract

Excessive glucose intake can lead to fat accumulation which can increase the aromatization of androgens to estrogen levels. High estrogen levels can disturb GnRH secretion feedback, prolonging the reproductive cycle, and affecting the uterine. As a condition, it is essential to encourage moderate exercise to reduce fat accumulation and restore GnRH function. Good physical exercise has sufficient rest duration so that the required intensity, frequency, and time are right. This study aims to see how glucose intake affects the body and uterine weight with or without moderate exercise. This research was an experimental study. Healthy female mice, divided into 3 groups, negative control, high-calorie diet, and high-calorie diet with moderate exercise. A highcalorie diet was given by using sonde dextrose 40% as much as 0,325 ml/g Body Weight. Moderate exercise was swimming with a load of 6% BW. Mice were swum together 3 times a week for 4 weeks. The duration of the first week of swimming was 5 minutes, the second week was 7 minutes, the third week was 11 minutes and the fourth week was 15 minutes. The result of this research was the uterus weight (p=0,051) did not show a significant difference between groups. However, uterus weight in the high-calorie diet group was higher (0,19gr) than a high-calorie diet with a moderate exercise group (0,14 gr) and negative control group (0,11 g). The vagina swab result (p=0,045) that there was a significant relationship between groups. Therefore, glucose intake with or without moderate exercise affects the reproductive cycle but did not affect uterine weight.