MTT-BASED CYTOTOXIC EVALUATION OF AMBONESE BANANA STEM SAP (Musa paradisiaca var. sapientum (L.) Kuntze) ON FIBROBLAST CELLS

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY

Periodico Tche Quimica Pub Date : 2020-12-20 DOI:10.52571/ptq.v17.n36.2020.639_periodico36_pgs_624_633.pdf

H. Budi, W. S. Juliastuti, Winda Ariani

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引用次数: 2

Abstract

Traditional herbal remedies are naturally occurring, plant-derived substances with limited to no chemical processing and have been used in local or national healing traditions to treat illness. In global health debates, traditional herbal medicines are gaining considerable attention. Many hope new research into herbal medicine will play a vital role in global health. Countries like China, India, Nigeria, USA, and also the World Health Organization (WHO) made large investments in ancient herbal medicines. Currently, the use of the Indonesian plant has dramatically improved the medical and dentistry field. The dental and oral care is often related to wounds, and the Ambonese banana stem has been proven as an effective treatment for these injuries. This study aimed to evaluate the effectiveness and safety of the Ambonese banana stem sap through cytotoxicity tests on the fibroblast cell culture of Baby Hamster Kidney-21 (BHK-21). This study was carried out on three BHK-21 fibroblast cell culture, namely, the media and cell control, and the Ambonese banana stem sap with a concentration of 10%, 20%, 30%, 40%, 50%, 60 %, 70%, 80%, 90%, and 100% incubated for 24 hours at 37ºC and 5% CO2. Then, MTT was evenly dispersed on the media to obtain accurate optical density value. All quantitative data were statistically analyzed using one-way ANOVA and Tukey's HSD Test. The result showed significant differences in optical density values between groups with p = 0.000 (p 0.05). There was no significant difference between the cell control and the Ambonese banana stem sap group with concentrations of 90%, 80%, 70%, 60%, 50%, 30%, 20%, and 10%. It was also observed that the Ambonese banana stem sap is nontoxic to fibroblast cells since its viability value was more than 60%.

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基于MTT的香蕉琥珀茎SAP（Musa paradisiaca var.sapientum（L.）Kuntze）对成纤维细胞的细胞毒性评价

传统草药是自然产生的植物衍生物质，很少或没有化学加工，在地方或国家的治疗传统中用于治疗疾病。在全球卫生辩论中，传统草药正获得相当大的关注。许多人希望草药的新研究将在全球健康中发挥至关重要的作用。中国、印度、尼日利亚、美国和世界卫生组织(WHO)等国家对古代草药进行了大量投资。目前，印度尼西亚植物的使用极大地改善了医疗和牙科领域。牙齿和口腔护理通常与伤口有关，而Ambonese香蕉茎已被证明是治疗这些伤口的有效方法。本研究旨在通过对小仓鼠肾-21 (BHK-21)成纤维细胞培养的细胞毒性试验，评价Ambonese香蕉茎汁的有效性和安全性。本研究采用3种BHK-21成纤维细胞培养，即培养基和细胞对照，以及浓度为10%、20%、30%、40%、50%、60%、70%、80%、90%和100%的Ambonese香蕉茎汁，在37℃、5% CO2条件下培养24小时。然后将MTT均匀分散在介质上，得到准确的光密度值。所有定量资料采用单因素方差分析和Tukey’s HSD检验进行统计学分析。结果显示，两组间光密度值差异显著，p = 0.000 (p 0.05)。细胞对照与浓度为90%、80%、70%、60%、50%、30%、20%和10%的香蕉茎液组之间无显著差异。结果表明，Ambonese香蕉茎液对成纤维细胞无毒性，其活性值大于60%。

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Periodico Tche Quimica CHEMISTRY, MULTIDISCIPLINARY-

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