Expression of VDR-related lncRNAs in malignancies originated from salivary gland: A pilot study

IF 0.8 Q4 GENETICS & HEREDITY

Meta Gene Pub Date : 2021-12-01 DOI:10.1016/j.mgene.2021.100980

Soudeh Ghafouri-Fard , Saede Atarbashi-Moghadam , Vahid Kholghi-Oskooei , Asghar Ashrafi Hafez , Mohammad Taheri

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引用次数: 0

Abstract

Salivary gland tumors are complex neoplastic disorders with unidentified basis. Based on the importance of vitamin D receptor signaling in the pathogenesis of neoplastic conditions, we measured expression of some mRNA coding genes (VDR, CYP24A1 and CYP25B1) and long non-coding RNAs (SNHG16, SNHG6, LINC00346 and LINC00511) from this pathway in 42 paraffin-embedded blocks from 37 patients with different disorders originated from salivary gland. Total RNA was extracted from these samples using commercial kits and expression of mentioned genes was measured in these samples using quantitative real time PCR method. Expression of LINC00511 was lower in tumor samples compared with pleomorphic adenoma (PA) samples (Expression ratio (ER) = 0.08, P value = 0.009). Yet, its expression was higher in PA samples compared with non-cancerous tissues adjacent to carcinoma samples (ER = 21.77, P = 0.009). In addition, its expression was lower in both adenoid cystic carcinoma and mucoepidermoid carcinoma samples compared with PA samples (ER = 0.11, P value = 0.046 and ER = 0.07, P value = 0.017, respectively). Expression of other genes was statistically similar between different lesions. Therefore, LINC00511 might be used as a marker for separation of salivary gland carcinomas from PA samples.

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涎腺恶性肿瘤中vdr相关lncrna的表达:一项初步研究

唾液腺肿瘤是一种基础不明的复杂肿瘤疾病。基于维生素D受体信号在肿瘤发病机制中的重要性，我们在37例不同涎腺疾病患者的42个石蜡包埋块中检测了该途径中部分mRNA编码基因(VDR、CYP24A1和CYP25B1)和长链非编码rna (SNHG16、SNHG6、LINC00346和LINC00511)的表达。使用商业试剂盒提取这些样品的总RNA，并使用定量实时PCR方法检测这些样品中上述基因的表达。与多形性腺瘤(PA)相比，LINC00511在肿瘤中的表达较低(表达比(ER) = 0.08, P值= 0.009)。但其在癌旁非癌组织中的表达高于癌旁非癌组织(ER = 21.77, P = 0.009)。此外，其在腺样囊性癌和黏液表皮样癌中的表达均低于PA (ER = 0.11, P值= 0.046,ER = 0.07, P值= 0.017)。其他基因在不同病变间的表达具有统计学上的相似性。因此，LINC00511可作为PA样本中唾液腺癌的分离标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.