Construction of a Dual Protease Column, Subzero (-30 °C) Chromatography System and Multi-channel Precision Temperature Controller for Hydrogen-Deuterium Exchange Mass Spectrometry

IF 1.3 4区工程技术 Q3 INSTRUMENTS & INSTRUMENTATION

Journal of Research of the National Institute of Standards and Technology Pub Date : 2020-08-12 DOI:10.6028/jres.125.025

J. W. Hudgens

引用次数: 4

Abstract

This tutorial provides mechanical drawings, electrical schematics, parts lists, stereolithography (STL) files for producing three-dimensional (3D)-printed parts, initial graphics exchange specification (IGS) files for automated machining, and instructions necessary for construction of a dual protease column, subzero, liquid chromatography system for hydrogen-deuterium exchange mass spectrometry (HDX-MS). Electro-mechanical schematics for construction of two multi-zone temperature controllers that regulate to ±0.05 oC are also included in this tutorial.

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双蛋白酶柱、亚零度（-30°C）色谱系统和多通道精密温度控制器的构建

本教程提供了用于生产三维（3D）打印零件的机械图纸、电气原理图、零件清单、立体光刻（STL）文件、用于自动加工的初始图形交换规范（IGS）文件，以及构建双蛋白酶柱subzero所需的说明，用于氢-氘交换质谱的液相色谱系统（HDX-MS）。本教程还包括两个可调节至±0.05℃的多区域温度控制器的机电原理图。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Research of the National Institute of Standards and Technology 工程技术-物理：应用

自引率

33.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Research of the National Institute of Standards and Technology is the flagship publication of the National Institute of Standards and Technology. It has been published under various titles and forms since 1904, with its roots as Scientific Papers issued as the Bulletin of the Bureau of Standards. In 1928, the Scientific Papers were combined with Technologic Papers, which reported results of investigations of material and methods of testing. This new publication was titled the Bureau of Standards Journal of Research. The Journal of Research of NIST reports NIST research and development in metrology and related fields of physical science, engineering, applied mathematics, statistics, biotechnology, information technology.