Toxicity effects evaluation of green synthesized silver nanoparticles on intraperitoneally exposed male Wistar rats

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2022-03-06 DOI:10.1080/15376516.2022.2049412

S. Tarbali, Saeed Karami Mehrian, Shiva Khezri

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引用次数: 3

Abstract

Abstract Objective: During the last decades, the widespread use of silver nanoparticles (AgNPs) has been considered because of their small size and antimicrobial effects. The main concern about these particles is that they can induce oxidative stress. In this study, the dose-dependent effects of green synthesized silver nanoparticles (Green-AgNPs) were evaluated on adult male rats. Methods: Animals were injected intraperitoneally (I.P) with the vehicle (deionized water) and different doses of Green-AgNPs (50, 100, 200, and 400 ppm), daily for 21 days. For the safety assessment, body weight and organ coefficient (liver, kidney, spleen, and brain) were measured. The effects of Green-AgNPs administration on working memory, anxiety behavior, novel object recognition, and spatial memory were analyzed. The lipophilic fluorescent products (LFPs), as an indicator of oxidative stress, were also evaluated in the liver, kidney, spleen, and hippocampus. Results: After 21 days of exposure, significant changes were not observed in body weight and organ coefficients. Green-AgNPs at the doses of 100, 200, and 400 ppm caused memory impairment and anxieties as well as altered liver, kidney, spleen, and hippocampus redox status. All tissues of the exposed animals showed an increased LFPs level compared to those of the rats in the vehicle group. Conclusions: This study indicated that the consumption of Green-AgNPs in higher doses (>50 ppm), not only had negative effects on behavioral indices but also caused memory impairment in rats and was toxic. This might be due to the induction of oxidative stress demonstrated by increased LFPs levels in tissues. Graphical Abstract Cascade events after I.P. injection of different doses of Green-AgNPs in rats After I.P. administration of green synthesized AgNPs in rats, these particles can pass through endothelial cells to various organs, such as the liver, kidney, spleen, and brain. Then caused oxidative stress and increased production of free radicals in the tissues. Overload free radicals result in the initiation of lipid peroxidation and result in the production of aldehydes. Following changes in the redox state in the neurons, these events caused memory impairment in high doses of green synthesized AgNPs. Aldehydes produced are unstable and react with amino groups of proteins, amino acids, or phospholipids. Assessment of generated lipophilic fluorescent products (LFPs) as stress oxidative marker.

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绿色合成纳米银对雄性Wistar大鼠腹腔注射的毒性作用评价

摘要目的:在过去的几十年里，银纳米颗粒(AgNPs)由于其小尺寸和抗菌作用而被广泛应用。对这些颗粒的主要担忧是它们会引起氧化应激。在本研究中，研究了绿色合成银纳米颗粒(green - agnps)对成年雄性大鼠的剂量依赖性。方法:动物腹腔注射不同剂量的Green-AgNPs(50、100、200、400 ppm)和去离子水，连续注射21 d。为了进行安全性评估，测量了体重和器官系数(肝、肾、脾和脑)。分析Green-AgNPs给药对工作记忆、焦虑行为、新物体识别和空间记忆的影响。作为氧化应激指标的亲脂性荧光产物(LFPs)也在肝脏、肾脏、脾脏和海马中进行了评估。结果:暴露21天后，大鼠体重和脏器系数无明显变化。100,200和400ppm剂量的绿色agnps引起记忆障碍和焦虑，以及肝脏，肾脏，脾脏和海马氧化还原状态的改变。与车辆组大鼠相比，暴露动物的所有组织均显示lfp水平升高。结论:大鼠大剂量(50 ~ 50 ppm)摄入绿agnps不仅对大鼠的行为指标有负面影响，还会引起记忆障碍，并具有毒性。这可能是由于组织中lfp水平增加所证明的氧化应激的诱导。大鼠ig注射不同剂量的绿色合成AgNPs后，这些颗粒可以通过内皮细胞到达肝、肾、脾、脑等各器官。然后引起氧化应激并增加组织中自由基的产生。过量的自由基导致脂质过氧化的开始，并导致醛的产生。随着神经元氧化还原状态的变化，这些事件导致高剂量绿色合成AgNPs的记忆损伤。产生的醛是不稳定的，与蛋白质、氨基酸或磷脂的氨基发生反应。生成的亲脂性荧光产物(LFPs)作为应激氧化标志物的评价。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.