Identifikasi Hiu yang Diperdagangkan di Bali Menggunakan Metode DNA Barcoding dan Analisis Filogenetik

Rizaldi Cahya Bramasta, Elok Faiqoh, I. Hendrawan, Andrianus Sembiring, Ni Luh Astria Yusmalinda
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引用次数: 2

Abstract

Sharks are one of the keys for ecosystems balance in the ocean. Sharks as top predators have an important role to maintain the stability of the food chain in the ocean. The loss of sharks will have a major impact on the marine ecosystem, so the presence of sharks in the ocean must be protected from extinction. However, there are some activities such as sharks hunting and trading going on because of the high demand of sharks processed products. Bali is one of the locations of shark fishing and trading activities. The problem is the trade-in sharks have gone through the finning process, leaving only the body parts or fins which resulted in the difficulty of morphological identification process. An alternative method that can be used if molecular identification cannot be done is DNA Barcoding. DNA Barcoding means identifying all the animal species with an effective molecular approach applied to identify species. The identification results of DNA Barcoding of shark’s trade in Bali shows that there are four species of sharks have been sold, which is hammerhead sharks (Sphyrna lewini), thresher sharks (Alopias pelagicus), big eye thresher shark (Alopias superliciosus), silky sharks (Carcharinus falciformis). These sharks registered in the IUCN red list, Sphyrna lewini classified as threatened species, while the others (Alopias pelagicus, Alopias superciliosus and Carcharinus falciformis) classified as vulnerable species. In the international trade regulations on CITES, these four species come under the Appendix II. In national regulations, Alopias pelagicus, Alopias superciliosus, Sphyrna lewini have been regulated by the Ministerial regulations, while for Carcharinus falciformis, there are no national regulations yet.
巴厘岛交易鲨鱼的鉴定使用了一种DNA条形码和丝质分析的方法
鲨鱼是海洋生态系统平衡的关键之一。鲨鱼作为顶级捕食者,在维持海洋食物链的稳定方面发挥着重要作用。鲨鱼的消失将对海洋生态系统产生重大影响,因此必须保护海洋中鲨鱼的存在,防止其灭绝。然而,由于对鲨鱼加工产品的高需求,一些活动如鲨鱼狩猎和交易仍在进行。巴厘岛是鲨鱼捕捞和贸易活动的地点之一。问题是,鲨鱼的贸易经历了割鳍的过程,只留下身体部位或鳍,这导致了形态识别过程的困难。如果不能进行分子鉴定,可以使用另一种方法是DNA条形码。DNA条形码是指用有效的分子方法识别所有动物物种。巴厘岛鲨鱼贸易的DNA条形码鉴定结果显示,目前已出售的鲨鱼有四种,分别是锤头鲨(Sphyrna lewini)、长尾鲨(Alopias pelagicus)、大眼长尾鲨(Elopias superlicious)和丝滑鲨(Carcharinus faliformis)。这些鲨鱼在国际自然保护联盟红色名录中登记,斯芬克娜·勒维尼被列为受威胁物种,而其他鲨鱼(白鳍豚、白鳍豚和镰状尸)则被列为易危物种。在《濒危野生动植物种国际贸易公约》的国际贸易条例中,这四个物种属于附录二。在国家法规中,pelagicus、Alopias supersiliosus、Sphyrna lewini已受到部长级法规的监管,而对于恶性Carcharinus faliformis,目前还没有国家法规。
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