Effect of Nanocurcumin in Combination with Methotrexate on Telomerase Activity, NF-kb Expression, and Proliferation Index of Bewo Choriocarcinoma Cells

Abstract

Objectives: To determine the anti-cancer effect of nanocurcumin on choriocarcinoma. Methods: This study observes the telomerase activity, NF-κ B expression, and BrdU proliferation index in cultures of BeWo choriocarcinoma cell line (ATCC CCL-98) exposed to MTX and nanocurcumin in various doses. The sample used in this study consisted of 4 groups of BeWo cells that received a combination of MTX and nanocurcumin, and 2 other groups for positive and negative control. Results: There was a decrease in telomerase activity, NF-κ B expression, and BrdU proliferation index in the 4 treatment groups compared to the negative and positive control group (p ≤ 0.05). Conclusion: Nanocurcumin and MTX decrease telomerase expression, NF-κ B expression, and the BrdU proliferation index in choriocarcinoma BeWo cell line culture faster than MTX alone.