Control of shoot-tip necrosis during Argylia radiata in vitro multiplication

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-12-01 DOI:10.1590/2447-536x.v28i4.2536

P. Morales-Tapia, M. Gambardella

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Abstract

Abstract Argylia radiata is an herbaceous perennial plant native to northern Chile and a representative species of the “Blooming Desert”. Due to its showy flowers and other morphological characteristics, A. radiata has great ornamental potential. In earlier work, a deep morpho-anatomical description was made, but the micropropagation protocols, which could be used for commercial purposes, are not known. Previous assays showed that cytokinin supplementation improves the multiplication rate but produces shoot-tip necrosis in the microplants. To avoid it, different modifications of the growth medium were tested, including calcium nitrate supplementation; increasing in agar concentration; indole-3-butyric acid enrichment; and change of the basal medium formulation. The effect of these changes over the damage level, number of shoots, multiplication rate, plant height (cm), fresh weight and dry weight (g), and water content (%) of the microplants were evaluated. The use of McCown Woody Plant formulation as basal medium showed the best effect, reducing the damage level and improving the multiplication rate. Additionally, IBA supplementation was effective in reducing necrotic damage. However, 0.1 mg L-1 of IBA significantly decreased the multiplication rate, while 0.01 mg L-1 led to a higher multiplication rate than that of plants grown in the control medium. In conclusion, the use of McCown Woody Plant medium and IBA supplementation should be considered in commercial A. radiata micropropagation.

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辐射银芽体外增殖过程中茎尖坏死的控制

摘要辐射银兰(Argylia radiata)是原产于智利北部的多年生草本植物，是“盛开的沙漠”的代表物种。由于其华丽的花朵和其他形态特征，辐射草具有很大的观赏潜力。在早期的工作中，进行了深入的形态解剖描述，但可用于商业目的的微繁殖协议尚不清楚。先前的实验表明，补充细胞分裂素可以提高微植株的增殖率，但会导致茎尖坏死。为了避免这种情况，研究人员对培养基进行了不同的修改，包括添加硝酸钙;琼脂浓度增加;吲哚-3-丁酸富集;基本培养基配方的变化。评价了这些变化对危害程度、芽数、增殖率、株高(cm)、鲜重和干重(g)、含水量(%)的影响。以麦黄木本配方为基础培养基效果最佳，可降低病害程度，提高增殖率。此外，补充IBA可有效减少坏死损伤。然而，0.1 mg L-1的IBA显著降低了植株的增殖率，而0.01 mg L-1的IBA使植株的增殖率高于对照培养基。综上所述，在商业放养中，应考虑使用McCown木本植物培养基和添加IBA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.