Clavulanic Acid - Susceptible Peptide Spectra Tested with Agar Disk Diffusion and Epsilometer test of Urinary Tract Infected Escherichia coli Isolated from the Community-Acquired Infection

Q3 Health Professions
Pitchaya Singhavejsakul, Piyahatai Niamsup, Naruenan Malairungsakul, Yosita Chaisaen, Phurich Pooriwarangkakul, W. Nimlamool, P. Khamnoi, T. Sastraruji, S. Sookkhee
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Abstract

Abstract The present study aimed to investigate clavulanic acid susceptible peptide spectra in 174 community-acquired urinary tract infected Escherichia coli isolates using VITEK® MS. They were collected from bacteriuria samples at Diagnostic Laboratory Unit between January and May 2016. One hundred and fourteen multidrug resistant isolates were detected. Among these isolates, 13, 53, and 48 isolates were classified as the non, high, and low ESBL-producers, respectively by using Epsilometer strip test. Spectrum analysis was calculated for investigating the enzyme spectrum. The mass analysis and spectrum identification of ESBL-producing isolates were elucidated using the VITEK® MS apparatus. Among 58 peptide spectra, seven E. coli-specific spectra located at 3126, 3433, 6317, 6411, 7159, 9537, and 10301 Dalton (Da) were commonly detected in ≥ 85% of the total samples. Among the high ESBL-producing isolates, the spectrum intensity of four ceftazidime-resistant spectra which were located at 4613, 5613, 6508, 9713 Da was significantly increased after being exposed to ceftazidime as the ceftazidime-resistant spectra. Using agar disk diffusion, four clavulanic acid-susceptible spectra, 3433, 4613, 4768, and 9713 Da, were significantly detected after being co-exposed to ceftazidime and amoxicillin/clavulanic acid. Interestingly, the spectrum intensity of the significant spectrum, 9713 Da, was decreased after being co-exposed to ceftazidime plus clavulanic acid using an Epsilometer strip test. The interesting peptide spectra at 9713 Da significantly exhibited the clavulanic acid-susceptibility after being co-exposed to ceftazidime and clavulanic acid in both assays and could be suspected to be the ESBL indicative peptide spectra in the high ESBL-producing isolates. Keywords: VITEK® MS, Escherichia coli, ESBL, Clavulanic acid-susceptible peptide spectra, Community-acquired urinary tract infection
社区获得性感染尿路感染大肠埃希菌的克拉维酸敏感肽谱的琼脂盘扩散和Epsilometer试验
摘要本研究旨在使用VITEK®MS研究174个社区获得性尿路感染大肠杆菌分离株的棒酸敏感肽谱。这些分离株是从2016年1月至5月诊断实验室的细菌尿样本中收集的。检测到一百一十四株多药耐药菌株。在这些分离株中,通过Epsilometer条带试验,分别将13株、53株和48株分离株分为非ESBL、高ESBL和低ESBL生产者。计算光谱分析以研究酶的光谱。使用VITEK®MS仪器对产ESBL的分离株进行了质量分析和光谱鉴定。在58个肽谱中,7个大肠杆菌特异性谱位于3126、3433、6317、6411、7159、9537和10301道尔顿(Da),在≥85%的样品中普遍检测到。在产ESBL较高的分离株中,位于4613、5613、6508、9713 Da的四个头孢他啶耐药谱的谱强度在暴露于头孢他啶后显著增加,作为头孢他啶的耐药谱。使用琼脂盘扩散法,在共同暴露于头孢他啶和阿莫西林/克拉维酸后,显著检测到四个克拉维酸敏感谱3433、4613、4768和9713 Da。有趣的是,在使用Epsilometer条带测试共同暴露于头孢他啶加克拉维酸后,有效光谱的光谱强度9713Da降低。在两种测定中,9713Da的有趣肽谱在共同暴露于头孢他啶和克拉维酸后显著表现出克拉维酸敏感性,并且可以怀疑是高ESBL产生分离株中的ESBL指示肽谱。关键词:VITEK®MS、大肠杆菌、ESBL、克拉维酸敏感肽谱、社区获得性尿路感染
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来源期刊
Chiang Mai University journal of natural sciences
Chiang Mai University journal of natural sciences Health Professions-Health Professions (miscellaneous)
CiteScore
1.70
自引率
0.00%
发文量
67
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