Seed fungal endophytes promote the establishment of invasive Poa annua in maritime Antarctica

Abstract

ABSTRACT Background Invasive plants may displace native species. This is the case for Poa annua, the only non-native plant species successfully established in Maritime Antarctica. Nonetheless, it is uncertain which factors drive the competitive success of P. annua in the harsh environmental conditions of the region. The ability of this plant species to establish novel mutualistic interactions with resident soil fungi may be crucial for its invasiveness. Such ability may be linked to the vertical transmission of fungal endophytes via seeds. Aims We undertook a study to assess the role of seed fungal endophytes as promoters of the establishment and invasion of Poa annua in Maritime Antarctica. Methods We explored the composition and diversity of fungal communities associated with different P. annua tissues (seeds, leaves and roots) and the soil. We also measured parameters including germination rate, above-ground biomass, reproductive structures, and the survival of invasive P. annua as well as of the native Colobanthus quitensis and Deschampsia antarctica grown from seeds with and without endophytes. Furthermore, we conducted inter- and intraspecific competition experiments among native and invasive plants, where chemically mediated plant-to-plant interference (allelopathy) and plant growth rate were measured to calculate a relative competition index. Results We found that fungal endophyte taxa associated with P. annua tissues were very different from those in the soil. Fungal endophytes in P. annua differed among seed, root and shoot tissues, which suggests low transmission among different organs. The removal of endophytes from P. annua seeds was associated with reduced seed germination, plant growth and survivorship, while the competitive ability of P. annua (assessed by accumulated biomass) relative to native species, as well as levels of allelochemicals in soils, were higher in the presence of seed fungal endophytes. Conclusion Our results suggest that fungal endophytes, maternally inherited through seeds, improve host fitness and may contribute to the invasive success of P. annua in Antarctica.