Adhesion, integration and osteogenesis of human dental pulp stem cells on biomimetic implant surfaces combined with plasma derived products.

IF 3.2 3区 医学 Q3 CELL & TISSUE ENGINEERING
I. Irastorza, J. Luzuriaga, R. Martínez‐Conde, G. Ibarretxe, F. Unda
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引用次数: 15

Abstract

Dental implants are the usual therapy of choice in the dental clinic to replace a loss of natural teeth. Over recent decades there has been an important progress in the design and manufacturing of titanium implant surfaces with the goal of improving their osteointegration. In the present work, the aim was to evaluate the usefulness of hDPSCs (human dental pulp stem cells), in combination with autologous plasma components, for in vitro bone generation on biomimetic titanium dental implant materials. In this context, the combination of hDPSCs stimulated by PRGF or PRF and cultured on standard Ti6A14V and biomimetic BAS™ (Avinent Implant System) titanium surfaces were studied in order to evaluate possible enhancements in the osteoblastic differentiation process out of human mesenchymal cells, as well as bone matrix secretion on the implant surface. The results obtained in this in vitro model of osteogenesis suggested a combination of biomimetic rough titanium surfaces, such as BAS™, with autologous plasma-derived fibrin-clot membranes such as PRF and/or insoluble PRGF formulations, but not with an addition of water-soluble supplements of plasma-derived growth factors, to maximise osteoblastic cell differentiation, bone generation, anchorage and osteointegration of titanium-made dental implants.
人牙髓干细胞与血浆衍生产物在仿生种植体表面的粘附、整合和成骨。
在牙科诊所,种植牙是替代天然牙齿缺失的常用疗法。近几十年来,在钛种植体表面的设计和制造方面取得了重要进展,其目标是改善其骨整合。在本工作中,目的是评估hDPSCs(人牙髓干细胞)结合自体血浆成分在仿生钛牙种植材料上体外成骨的有效性。在这种情况下,研究了PRGF或PRF刺激hDPSCs并在标准Ti6A14V和仿生BAS™(Avinent Implant System)钛表面培养的组合,以评估可能增强人间充质细胞成骨分化过程以及种植体表面骨基质分泌的可能性。在这个体外成骨模型中获得的结果表明,将仿生粗糙钛表面(如BAS™)与自体血浆源性纤维蛋白凝块膜(如PRF和/或不溶性PRGF配方)结合,但不添加血浆源性生长因子的水溶性补充剂,以最大限度地提高成骨细胞分化、骨生成、钛制牙种植体的锚定和骨整合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
European cells & materials
European cells & materials 生物-材料科学:生物材料
CiteScore
6.00
自引率
6.50%
发文量
55
审稿时长
1.5 months
期刊介绍: eCM provides an interdisciplinary forum for publication of preclinical research in the musculoskeletal field (Trauma, Maxillofacial (including dental), Spine and Orthopaedics). The clinical relevance of the work must be briefly mentioned within the abstract, and in more detail in the paper. Poor abstracts which do not concisely cover the paper contents will not be sent for review. Incremental steps in research will not be entertained by eCM journal.Cross-disciplinary papers that go across our scope areas are welcomed.
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