Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves

IF 0.6 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Bioscience Journal Pub Date : 2023-01-27 DOI:10.14393/bj-v39n0a2023-62323

Frank Bruno Vieira de Sousa, Á. Cruz, Daniela Francisca Soares, C. Cunha, J. Holzbach

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Abstract

Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.

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眼镜叶和桂树叶的化学成分及体内毒理学活性初步评价

尽管文献中很少有化学和生物学研究报道，但巴西塞拉多地区发现的阴蒂和乌拉特拉(Ouratea spectabilis)被用于民间医学。本研究旨在研究两种植物提取物对微甲壳类动物盐蒿的毒性和作用，并测定赤叶草(O. spectabilis)叶己烷提取物和赤叶草(C. guianensis)叶乙酸乙酯组分的化学成分。从桂树叶乙酸乙酯中分离得到了一种类黄酮——山柰素。采用气相色谱-质谱联用技术对黄叶己烷提取物进行分析，发现其中含有25种已知物质。桂草叶的Hex、EtOAc和EtOH粗提物对盐蒿具有高、中毒性，致死剂量中值(LD50)分别为43.7、25.4和233.4 mg。分别L−1。黄花叶丙酮提取物对盐渍蒿具有中等毒性，LD50值为115.13 mg.L−1。

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来源期刊

Bioscience Journal Agricultural and Biological Sciences-General Agricultural and Biological Sciences

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

48 weeks

期刊介绍： The Bioscience Journal is an interdisciplinary electronic journal that publishes scientific articles in the areas of Agricultural Sciences, Biological Sciences and Health Sciences. Its mission is to disseminate new knowledge while contributing to the development of science in the country and in the world. The journal is published in a continuous flow, in English. The opinions and concepts expressed in the published articles are the sole responsibility of their authors.