The wound healing effect of allogeneic freeze-dried platelet-rich plasma in a full-thickness wound animal model

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
I. Saputro, A. Ardan, W. Yuniarti, O. Putra
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引用次数: 1

Abstract

Background: Epithelialization is an indicator of wound healing. Platelet-rich plasma (PRP) may accelerate the epithelialization of the wound due to high amount of growth factors. Generally, allogeneic PRP provides a smaller immunological effect than autologous PRP. By a freeze-drying method, it is assumed that allogeneic PRP has lower allergenic activity. Aims and Objectives: The aim of this study was to investigate the effect of allogeneic freeze-dried PRP on wound healing of a full-thickness wound in New Zealand rabbits. About 2 × 2 cm2 full-thickness wounds were created on rabbits using a template on both sides of the dorsum and divided into treatment group and control group. The treatment group was treated with allogeneic freeze-dried PRP and the control group was treated with moist dressing. Acceleration of wound healing was shown by the epithelialization and measured on day 7 using digital Visitrak. Nine New Zealand rabbits were used in this study. Results: In the treatment group, the epithelialization was significantly higher 3.00 ± 0.96 cm2 than the control group 1.35 ± 0.85 cm2 (P < 0.000) by independent t test. Another finding of our study was the allergic reaction was not observed after the administration of allogeneic freeze-dried PRP in rabbits. Conclusion: Our results indicate that allogeneic freeze-dried PRP accelerates epithelialization compared to the control group and does not cause an allergic reaction in full-thickness wounds in rabbits. The effect of allogeneic PRP, prepared with a freeze-drying method, on the process of wound healing is reported for the first time in this article.
同种异体冻干富血小板血浆在全层创伤动物模型中的创伤愈合作用
背景:上皮化是伤口愈合的一个指标。富含血小板的血浆(PRP)可能由于大量的生长因子而加速伤口的上皮化。一般来说,同种异体PRP比自体PRP提供更小的免疫效果。通过冷冻干燥方法,假定同种异体PRP具有较低的致敏活性。目的:本研究旨在研究同种异体冻干PRP对新西兰兔全层伤口愈合的影响。用模板在兔背两侧造成约2×2 cm2的全厚伤口,分为治疗组和对照组。治疗组采用异基因冻干PRP治疗,对照组采用湿润敷料治疗。通过上皮化显示伤口愈合的加速,并在第7天使用数字Visitrak进行测量。本研究使用了9只新西兰兔子。结果:经独立t检验,治疗组的上皮化面积为3.00±0.96cm2,明显高于对照组的1.35±0.85cm2(P<0.000)。我们研究的另一个发现是,在兔子中施用同种异体冻干PRP后没有观察到过敏反应。结论:我们的研究结果表明,与对照组相比,同种异体冻干PRP加速了兔全层伤口的上皮化,并且不会引起过敏反应。本文首次报道了冷冻干燥法制备的异基因PRP对伤口愈合过程的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Reports in Pharmaceutical Sciences
Journal of Reports in Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.40
自引率
0.00%
发文量
0
期刊介绍: The Journal of Reports in Pharmaceutical Sciences(JRPS) is a biannually peer-reviewed multi-disciplinary pharmaceutical publication to serve as a means for scientific information exchange in the international pharmaceutical forum. It accepts novel findings that contribute to advancement of scientific knowledge in pharmaceutical fields that not published or under consideration for publication anywhere else for publication in JRPS as original research article. all aspects of pharmaceutical sciences consist of medicinal chemistry, molecular modeling, drug design, pharmaceutics, biopharmacy, pharmaceutical nanotechnology, pharmacognosy, natural products, pharmaceutical biotechnology, pharmacology, toxicology and clinical pharmacy.
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