Selection of redox dye and inoculum conditions for the optimisation of respirometric indices in Verticillium and Trichoderma

IF 2 4区农林科学 Q2 AGRONOMY

International Agrophysics Pub Date : 2023-08-28 DOI:10.31545/intagr/170884

K. Oszust, F. Pinzari, M. Frąc

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引用次数: 0

Abstract

. Understanding which carbon or nitrogen sources the competing fungal species prefer is pivotal for specific biotechnological applications. However, this is not straightforward, as each strain sometimes behaves differently under the experimental conditions adopted. To analyse the trophic overlap of two species, it is necessary to refine diagnostic techniques and exclude variables that may interfere with the measurements. A protocol for establishing the suitability of chromogenic dyes in the analysis of filamentous fungi with phenotype microarrays is described here. The research goal was to determine the most suitable redox dye indicator and its optimal concentration that reacts quantitatively to the respiratory activity of both Verticillium spp. and Trichoderma spp. isolates in the presence of a nitrogen source. The commercial Biolog TM Redox Dye Mixes D, E, and F and also TTC (2,3,5-Triphenyltetrazolium chloride), INT (2-(4-iodophenyl)- 3-(4-nitrophenyl)-5-phenyl-2 H -tetrazolium chloride), and MTT solutions were evaluated in selected concentrations. Both their nutritive and toxic effects were quantified. Results showed that 1% “F” and 0.5% “D” Biolog TM dyes were appropriate for the functional evaluation of isolates belonging to the Verticillium and Trichoderma genera. They can be used in Biolog TM PM-nitrogen plate respiratory assays for a comprehensive functional charac - terisation of these organisms.

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氧化还原染料的选择及接种条件对黄萎病菌和木霉呼吸指标的优化

。了解竞争真菌物种更喜欢哪种碳源或氮源对于特定的生物技术应用至关重要。然而，这并不简单，因为每个菌株在所采用的实验条件下有时表现不同。为了分析两个物种的营养重叠，有必要改进诊断技术并排除可能干扰测量的变量。本文描述了一种方案，用于在丝状真菌的表型微阵列分析中建立显色染料的适用性。研究的目的是确定最合适的氧化还原染料指示剂及其最佳浓度，以定量影响氮源存在下黄萎病菌和木霉分离株的呼吸活性。对商业生物TM氧化还原染料混合物D、E和F以及TTC(2,3,5-三苯基氯化四氮唑)、INT(2-(4-碘苯基)- 3-(4-硝基苯基)-5-苯基-2 H氯化四氮唑)和MTT溶液在选定浓度下进行了评估。量化了它们的营养和毒性作用。结果表明，1%的“F”和0.5%的“D”biologic TM染料对黄萎病菌属和木霉属分离株的功能评价是合适的。它们可用于生物TM - pm -氮板呼吸分析，用于这些生物的全面功能表征。

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来源期刊

International Agrophysics 农林科学-农艺学

CiteScore

3.60

自引率

9.10%

发文量

审稿时长

3 months

期刊介绍： The journal is focused on the soil-plant-atmosphere system. The journal publishes original research and review papers on any subject regarding soil, plant and atmosphere and the interface in between. Manuscripts on postharvest processing and quality of crops are also welcomed. Particularly the journal is focused on the following areas: implications of agricultural land use, soil management and climate change on production of biomass and renewable energy, soil structure, cycling of carbon, water, heat and nutrients, biota, greenhouse gases and environment, soil-plant-atmosphere continuum and ways of its regulation to increase efficiency of water, energy and chemicals in agriculture, postharvest management and processing of agricultural and horticultural products in relation to food quality and safety, mathematical modeling of physical processes affecting environment quality, plant production and postharvest processing, advances in sensors and communication devices to measure and collect information about physical conditions in agricultural and natural environments. Papers accepted in the International Agrophysics should reveal substantial novelty and include thoughtful physical, biological and chemical interpretation and accurate description of the methods used. All manuscripts are initially checked on topic suitability and linguistic quality.